{"id":35,"date":"2020-05-20T21:22:37","date_gmt":"2020-05-20T21:22:37","guid":{"rendered":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/?page_id=35"},"modified":"2024-11-13T23:26:51","modified_gmt":"2024-11-13T23:26:51","slug":"protocols","status":"publish","type":"page","link":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"<div id='full_slider_1'  class='avia-fullwidth-slider main_color avia-shadow   avia-builder-el-0  el_before_av_heading  avia-builder-el-first   container_wrap fullsize'  ><div  class='avia-slideshow av-ky7znvdr-e69d5307cda80a3be6b7865e92288fc3 avia-slideshow-featured av_slideshow_full avia-slide-slider av-slideshow-ui av-control-default av-slideshow-manual av-loop-once av-loop-manual-endless av-default-height-applied   avia-slideshow-1' data-slideshow-options=\"{&quot;animation&quot;:&quot;slide&quot;,&quot;autoplay&quot;:false,&quot;loop_autoplay&quot;:&quot;once&quot;,&quot;interval&quot;:5,&quot;loop_manual&quot;:&quot;manual-endless&quot;,&quot;autoplay_stopper&quot;:false,&quot;noNavigation&quot;:false,&quot;bg_slider&quot;:false,&quot;keep_padding&quot;:false,&quot;hoverpause&quot;:false,&quot;show_slide_delay&quot;:0}\"  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><ul class='avia-slideshow-inner ' style='padding-bottom: 28.666666666667%;'><li  class='avia-slideshow-slide av-ky7znvdr-e69d5307cda80a3be6b7865e92288fc3__0  av-single-slide slide-1 slide-odd'><div data-rel='slideshow-1' class='avia-slide-wrap '   ><img decoding=\"async\" fetchpriority=\"high\" class=\"wp-image-1118 avia-img-lazy-loading-not-1118\"  src=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2022\/01\/PXL_20211002_162827860-1500x430.jpg\" width=\"1500\" height=\"430\" title='PXL_20211002_162827860' alt=''  itemprop=\"thumbnailUrl\"   \/><\/div><\/li><\/ul><\/div><\/div><div id='after_full_slider_1'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-35'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-uuwn-a92fee67914db72655bf140072c11e85\">\n#top .av-special-heading.av-uuwn-a92fee67914db72655bf140072c11e85{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-uuwn-a92fee67914db72655bf140072c11e85 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-uuwn-a92fee67914db72655bf140072c11e85 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-uuwn-a92fee67914db72655bf140072c11e85 av-special-heading-h3 blockquote modern-quote modern-centered  avia-builder-el-1  el_after_av_slideshow_full  el_before_av_textblock  avia-builder-el-first  av-linked-heading'><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >MATLAB CODE FOR ANALYZING TIFF FILES FROM 3I SLIDEBOOK SOFTWARE<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n<section  class='av_textblock_section av-kaii57vp-e074e99b2293103ae7a62b48a6799935 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock protocol_first_paragraph'  itemprop=\"text\" ><p style=\"text-align: left\"><a href=\"https:\/\/github.com\/bjhardcastle\/SlidebookObj\" target=\"_blank\" rel=\"noopener\">SlidebookObj <\/a>is a Matlab class for processing and analyzing Tiff files generated by the Frye lab 3i 2-photon system. The class contains some basic tools for exploring data using ROIs (similar to the Slidebook software), making use of the accompanying DAQ file saved with each experiment to make it easy to find sections of a recording which correspond to a particular stimulus event.<\/p>\n<\/div><\/section>\n<div  class='hr av-63kkv-de107009e437197b6941767741f1972b hr-default  avia-builder-el-3  el_after_av_textblock  el_before_av_heading '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-297bv-dd9cdc17f096548ce4bd57bfd9f77b86\">\n#top .av-special-heading.av-297bv-dd9cdc17f096548ce4bd57bfd9f77b86{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-297bv-dd9cdc17f096548ce4bd57bfd9f77b86 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-297bv-dd9cdc17f096548ce4bd57bfd9f77b86 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-297bv-dd9cdc17f096548ce4bd57bfd9f77b86 av-special-heading-h3 blockquote modern-quote modern-centered  avia-builder-el-4  el_after_av_hr  el_before_av_image  av-linked-heading'><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >DUAL M-SEQUENCE VISUAL SPATIO-TEMPORAL ACTION FIELD (STAF)<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-kaii9g8y-4dc16f99ab87e0e2e3b6f8dc24ac36c1\">\n.avia-image-container.av-kaii9g8y-4dc16f99ab87e0e2e3b6f8dc24ac36c1 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-kaii9g8y-4dc16f99ab87e0e2e3b6f8dc24ac36c1 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-kaii9g8y-4dc16f99ab87e0e2e3b6f8dc24ac36c1 av-styling- avia-align-center  avia-builder-el-5  el_after_av_heading  el_before_av_textblock '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-279 avia-img-lazy-loading-not-279 avia_image ' src=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/stafpic_orig-300x225.jpg\" alt='Dual M-Sequence Visual Spatio-Temporal Action Field Graph' title=''  height=\"225\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/stafpic_orig-300x225.jpg 300w, https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/stafpic_orig-768x576.jpg 768w, https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/stafpic_orig-624x468.jpg 624w, https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/stafpic_orig.jpg 960w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div>\n<section  class='av_textblock_section av-kaii9qvw-66b2504390ffee27f874a8c1d7fd0ba6 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><p style=\"text-align: center\">The paper describing the technique is\u00a0<a href=\"http:\/\/journal.frontiersin.org\/article\/10.3389\/fncir.2014.00130\/abstract\" target=\"_blank\" rel=\"noopener\">here<\/a>. \u00a0Annotated Matlab code (ZIP) can be downloaded\u00a0<a href=\"https:\/\/drive.google.com\/open?id=0B_Yl9iQajtghNzJnRUdnTFYtOVk\" target=\"_blank\" rel=\"noopener\">here<\/a>.\u00a0\u200b<\/p>\n<\/div><\/section>\n<div  class='hr av-4d8zf-b49adbeda29f9cf53f7db9a0ca6261eb hr-default  avia-builder-el-7  el_after_av_textblock  el_before_av_heading '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-1hu2f-b5059aa97115017ac7b1b59f802d95d9\">\n#top .av-special-heading.av-1hu2f-b5059aa97115017ac7b1b59f802d95d9{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-1hu2f-b5059aa97115017ac7b1b59f802d95d9 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-1hu2f-b5059aa97115017ac7b1b59f802d95d9 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-1hu2f-b5059aa97115017ac7b1b59f802d95d9 av-special-heading-h3 blockquote modern-quote modern-centered  avia-builder-el-8  el_after_av_hr  el_before_av_image  av-linked-heading'><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >FLIGHT SIMULATORS<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-kaiiawbh-a2d8d469e57a75e5ea0646a85ef1d36d\">\n.avia-image-container.av-kaiiawbh-a2d8d469e57a75e5ea0646a85ef1d36d img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-kaiiawbh-a2d8d469e57a75e5ea0646a85ef1d36d .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-kaiiawbh-a2d8d469e57a75e5ea0646a85ef1d36d av-styling- avia-align-center  avia-builder-el-9  el_after_av_heading  el_before_av_textblock '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-127 avia-img-lazy-loading-not-127 avia_image ' src=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/20160217-113525-1_orig-300x246.jpg\" alt='Magnetic tether rig' title=''  height=\"246\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/20160217-113525-1_orig-300x246.jpg 300w, https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/20160217-113525-1_orig-624x511.jpg 624w, https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/20160217-113525-1_orig.jpg 761w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div>\n<section  class='av_textblock_section av-kaiib6h5-582bb7e2a610215e87cd4c34888d1c32 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><p style=\"text-align: center\">Videos describing the magnetic tether method and experiments can be found\u00a0<a href=\"http:\/\/www.jove.com\/video\/1063\/a-magnetic-tether-system-to-investigate-visual-olfactory-mediated\">here\u00a0<\/a>and\u00a0<a href=\"http:\/\/www.jove.com\/video\/1110\/visually-mediated-odor-tracking-during-flight-in-drosophila\">here<\/a>.<br \/>\nThe modular LED panel architecture was published by Reiser and Dickinson\u00a0<a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0165027007003755\">here<\/a>\u00a0and can be purchased\u00a0<a href=\"https:\/\/iorodeo.com\/\" target=\"_blank\" rel=\"noopener\">here<\/a>.<br \/>\nParts lists for the arenas in our lab can be found\u00a0<a href=\"https:\/\/docs.google.com\/spreadsheets\/d\/1tTBZNTL9qb1LjBsi8TFxCn1jEPhV0w7TFkZ9Dg5q99U\/edit?usp=sharing\">here<\/a>\u00a0(thanks to Sara).<\/p>\n<\/div><\/section>\n<div  class='hr av-2jmhf-3360ada50ce8f7ce52aaada3e77d5801 hr-default  avia-builder-el-11  el_after_av_textblock  el_before_av_heading '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-xdkj-9eb3bc8c6afd48dcd87e46bf581fbf26\">\n#top .av-special-heading.av-xdkj-9eb3bc8c6afd48dcd87e46bf581fbf26{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-xdkj-9eb3bc8c6afd48dcd87e46bf581fbf26 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-xdkj-9eb3bc8c6afd48dcd87e46bf581fbf26 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-xdkj-9eb3bc8c6afd48dcd87e46bf581fbf26 av-special-heading-h3 blockquote modern-quote modern-centered  avia-builder-el-12  el_after_av_hr  el_before_av_one_full  av-linked-heading'><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >FUNNY<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n<div  class='flex_column av-u0kz-debfe18ed7abe2f3aa0bb89100e5dd4a av_one_full  avia-builder-el-13  el_after_av_heading  avia-builder-el-last  first flex_column_div  '     ><div  class='avia-video av-kaiibrl1-0d06595d34c6a95d7ffd4eac50b13f4a avia-video-16-9 av-small-hide av-mini-hide av-no-preview-image avia-video-load-always avia-video-html5'  itemprop=\"video\" itemtype=\"https:\/\/schema.org\/VideoObject\"  data-original_url='https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/1080p_419.mp4'><video class='avia_video' poster=\"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/1080p_419.jpg\"  loop preload=\"metadata\" muted controls id='player_35_715080150_2047040420'><source src='https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-content\/uploads\/sites\/19\/2020\/05\/1080p_419.mp4' type='video\/mp4' \/><\/video><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":664,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-35","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/pages\/35","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/users\/664"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/comments?post=35"}],"version-history":[{"count":27,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/pages\/35\/revisions"}],"predecessor-version":[{"id":1881,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/pages\/35\/revisions\/1881"}],"wp:attachment":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-frye\/wp-json\/wp\/v2\/media?parent=35"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}