{"id":10,"date":"2019-09-16T12:51:05","date_gmt":"2019-09-16T19:51:05","guid":{"rendered":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/?page_id=10"},"modified":"2023-08-18T13:35:25","modified_gmt":"2023-08-18T20:35:25","slug":"homepage-old","status":"publish","type":"page","link":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/homepage-old\/","title":{"rendered":"Homepage 2"},"content":{"rendered":"\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-q5c6-541cb8980fef5b3786158b6c18c137de\">\n.flex_column.av-q5c6-541cb8980fef5b3786158b6c18c137de{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-q5c6-541cb8980fef5b3786158b6c18c137de av_one_fourth  avia-builder-el-0  el_before_av_one_half  avia-builder-el-first  first flex_column_div av-zero-column-padding  '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-k0mxyz4t-de4116d6987edbdb1829f5542a707744\">\n.avia-image-container.av-k0mxyz4t-de4116d6987edbdb1829f5542a707744 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-k0mxyz4t-de4116d6987edbdb1829f5542a707744 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-k0mxyz4t-de4116d6987edbdb1829f5542a707744 av-styling-circle avia-align-center  avia-builder-el-1  el_before_av_image  avia-builder-el-first '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-104 avia-img-lazy-loading-not-104 avia_image ' src=\"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-content\/uploads\/sites\/224\/2019\/09\/Villanueva_Lab_August_2016_-22-1-e1568670554794-180x180.jpg\" alt='' title='Villanueva_Lab_August_2016_---22'  height=\"180\" 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id=\"style-css-av-k0mxtjw6-14bc4251100409d7202745104a0ff642\">\n.avia-image-container.av-k0mxtjw6-14bc4251100409d7202745104a0ff642 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-k0mxtjw6-14bc4251100409d7202745104a0ff642 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-k0mxtjw6-14bc4251100409d7202745104a0ff642 av-styling- av-hover-grow av-img-linked avia-align-center  avia-builder-el-2  el_after_av_image  el_before_av_image '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><a href=\"https:\/\/scholar.google.com\/citations?user=WHHb_MYAAAAJ&amp;hl=en\" class='avia_image '  target=\"_blank\"  rel=\"noopener noreferrer\" aria-label='Google_Scholar_logo'><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-96 avia-img-lazy-loading-not-96 avia_image ' 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id=\"style-css-av-k0mxu2w8-287795560d0f1b1698eb3b0399e3f694\">\n.avia-image-container.av-k0mxu2w8-287795560d0f1b1698eb3b0399e3f694 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-k0mxu2w8-287795560d0f1b1698eb3b0399e3f694 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-k0mxu2w8-287795560d0f1b1698eb3b0399e3f694 av-styling- av-hover-grow av-img-linked avia-align-center  avia-builder-el-3  el_after_av_image  avia-builder-el-last '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><a href=\"https:\/\/www.researchgate.net\/profile\/Claudio_Villanueva\" class='avia_image '  target=\"_blank\"  rel=\"noopener noreferrer\" aria-label='45e99a0d-f0ca-4b17-951a-ed0947f3fd0e-1510667426262'><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-97 avia-img-lazy-loading-not-97 avia_image ' 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https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-content\/uploads\/sites\/224\/2019\/09\/45e99a0d-f0ca-4b17-951a-ed0947f3fd0e-1510667426262-710x375.png 710w, https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-content\/uploads\/sites\/224\/2019\/09\/45e99a0d-f0ca-4b17-951a-ed0947f3fd0e-1510667426262-705x370.png 705w, https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-content\/uploads\/sites\/224\/2019\/09\/45e99a0d-f0ca-4b17-951a-ed0947f3fd0e-1510667426262-450x236.png 450w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/div><\/div><\/div><\/p><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-247oi-c79c4e8c9304d31ee7c374d800a787d5\">\n.flex_column.av-247oi-c79c4e8c9304d31ee7c374d800a787d5{\nborder-radius:0px 0px 0px 0px;\npadding:25px 25px 25px 25px;\nbackground-color:#f8f8f8;\n}\n<\/style>\n<div  class='flex_column av-247oi-c79c4e8c9304d31ee7c374d800a787d5 av_one_half  avia-builder-el-4  el_after_av_one_fourth  el_before_av_one_fourth  flex_column_div  '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-1ig9q-6a1299b02dead88ee1d89e3479534d80\">\n#top .av-special-heading.av-1ig9q-6a1299b02dead88ee1d89e3479534d80{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-1ig9q-6a1299b02dead88ee1d89e3479534d80 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-1ig9q-6a1299b02dead88ee1d89e3479534d80 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-1ig9q-6a1299b02dead88ee1d89e3479534d80 av-special-heading-h3  avia-builder-el-5  el_before_av_textblock  avia-builder-el-first '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >Research<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div><br \/>\n<section  class='av_textblock_section av-k0muau5v-dcbe5bb7659cb5025805ceecabedc480 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><div id=\"attachment_55\" style=\"width: 271px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-55\" class=\"size-full wp-image-55\" src=\"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-content\/uploads\/sites\/224\/2019\/09\/differentiation.jpg\" alt=\"Immunofluorescence of adipocytes differentiating in a tissue culture dish. Lipid droplets are stained green with Bodipy and nuclei are stained blue with DAPI.\" width=\"261\" height=\"222\" \/><p id=\"caption-attachment-55\" class=\"wp-caption-text\">Immunofluorescence of adipocytes differentiating in a tissue culture dish. Lipid droplets are stained green with Bodipy and nuclei are stained blue with DAPI.<\/p><\/div>\n<p class=\"Body\">We are interested in studying the transcriptional networks that regulate metabolism and how adipocytes establish their distinct metabolic programs. Adipocytes are specialized cells with a tremendous capacity to store energy in the form of triglycerides.\u00a0 There are three types of adipocytes, white, brown, and beige. White adipocytes store energy, while brown adipocytes consume energy for the highly energy demanding process of thermogenesis.\u00a0 Brown adipocytes are activated within minutes of cold exposure, while beige adipocytes are thermogenic like brown adipocytes, but appear in white adipose tissue with long-term cold exposure.\u00a0 In humans, brown fat activity is inversely correlated with body weight.\u00a0 Suggesting that brown fat plays a key role in contributing to whole body energy expenditure.\u00a0 Our laboratory will use imaging, biochemical, genetic, genomic, proteomic, and lipidomic approaches to study transcriptional regulators of adipocyte biology.<\/p>\n<p class=\"paragraph_style_3\"><em><strong>Transcriptional Regulation of Adipogenesis<\/strong><\/em><\/p>\n<p class=\"Body\">The central regulator of adipogenesis is PPAR\u03b3, a ligand activated transcription factor that belongs to the nuclear receptor family. PPAR\u03b3 is also the molecular target of thiazolidinediones (TZDs), a class of potent antidiabetic drugs. PPAR\u03b3 has a major role in driving adipocyte development and establishing the metabolic program of adipocytes. We recently identified TLE3 (transducin-like enhancer of split) as a transcriptional coregulator that works in concert with PPAR\u03b3 to drive adipocyte specific gene expression. We discovered that TLE3 and PPAR\u03b3 are involved in a feedforward loop and work synergistically to drive expression of genes involved in lipid handling and storage (Figure 1). Like TZD administration, overexpression of TLE3 in adipose tissue activates PPAR\u03b3 signaling and improves glucose handling and insulin sensitivity. Future investigations will examine the molecular crosstalk between TLE3 and PPAR\u03b3 signaling in adipocyte biology.<\/p>\n<p class=\"paragraph_style_3\"><em><strong>TLE3 and Wnt Signaling<\/strong><\/em><\/p>\n<p class=\"Body\">PPAR\u03b3 and Wnt signaling are opposing forces in adipogenesis. While PPAR\u03b3 drives adipogenesis, components of the Wnt signaling pathway oppose it. Genomewide association studies have identified polymorphisms in PPAR\u03b3 and TCF7L2 that strongly associate with type 2 diabetes. Although a wealth of data exists on how PPAR\u03b3 affects adipocyte biology and improves insulin sensitivity, little is known about the role of TCF7L2 in adipocyte development, endocrine function and energy metabolism. We discovered that during the course of adipogenesis expression of Wnt responsive genes inversely correlates with PPAR\u03b3 and TLE3 expression. We found that forced expression of TLE3 blocks Wnt-dependent gene expression and \u03b2-catenin-dependent reporter activity. Through a direct interaction TLE3 prevents the downregulation of adipogenic genes by Wnt3a. Notably, through TLE3 we identified a new mechanism that links PPAR\u03b3 to the inhibition of \u03b2-catenin-dependent Wnt signaling during the course of adipogenesis. Our lab will explore how transcriptional regulators of the Wnt signaling pathway modulate adipocyte biology.<\/p>\n<p class=\"paragraph_style_3\"><em><strong>Role of TLE3 in White and Brown Fat Cell Determination<\/strong><\/em><\/p>\n<p class=\"Body\">There are two types of adipocytes, white and brown. White adipocytes play a major role in storing lipid in the form of triglycerides, and mobilizing lipids as free fatty acids to provide an energy source for peripheral tissues such as heart, liver, and muscle. In contrast, brown adipocytes are thermogenic cells that express high levels of UCP1, a mitochondrial uncoupling protein that uncouples the proton gradient to generate heat. During cold exposure brown adipocytes catabolize lipids as an energy source for heat production. The molecular determinants that drive white versus brown fat gene expression are incompletely understood. Recently Prdm16 was identified as a critical determinant of brown adipocyte development. We are interested in investigating the crosstalk between TLE3 and Prdm16 in driving brown fat cell specification.<\/p>\n<p class=\"Body\">Our goal is to study transcriptional regulators of metabolism and adipocyte development, and how they relate to physiology and disease. Ultimately we hope to that our investigations will lead to novel therapeutics for the treatment of metabolic disorders such as obesity and type 2 diabetes.<\/p>\n<\/div><\/section><\/p><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-srci-9fd3a310c92032cc2f36fc712100e0e1\">\n.flex_column.av-srci-9fd3a310c92032cc2f36fc712100e0e1{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-srci-9fd3a310c92032cc2f36fc712100e0e1 av_one_fourth  avia-builder-el-7  el_after_av_one_half  avia-builder-el-last  flex_column_div av-zero-column-padding  '     ><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":126,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-10","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/pages\/10","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/users\/126"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/comments?post=10"}],"version-history":[{"count":19,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/pages\/10\/revisions"}],"predecessor-version":[{"id":505,"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/pages\/10\/revisions\/505"}],"wp:attachment":[{"href":"https:\/\/sites.lifesci.ucla.edu\/ibp-villanuevalab\/wp-json\/wp\/v2\/media?parent=10"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}