{"id":24,"date":"2017-04-19T12:38:26","date_gmt":"2017-04-19T19:38:26","guid":{"rendered":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/?page_id=24"},"modified":"2018-10-11T14:41:55","modified_gmt":"2018-10-11T21:41:55","slug":"protocols-reagents","status":"publish","type":"page","link":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/protocols-reagents\/","title":{"rendered":"Protocols &amp; Reagents"},"content":{"rendered":"<div class='flex_column_table av-6tgo4-bded6984c4e737f6665ebd2d5353c16d sc-av_three_fourth av-equal-height-column-flextable'>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-6tgo4-bded6984c4e737f6665ebd2d5353c16d\">\n.flex_column.av-6tgo4-bded6984c4e737f6665ebd2d5353c16d{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-6tgo4-bded6984c4e737f6665ebd2d5353c16d av_three_fourth  avia-builder-el-0  el_before_av_one_fourth  avia-builder-el-first  first no_margin flex_column_table_cell av-equal-height-column av-align-top av-zero-column-padding  '     ><div  class='avia-slideshow av-6crz4-8bc718b3dd7ccb72815dccf5412e448e avia-slideshow-no scaling av_slideshow avia-fade-slider  avia-builder-el-1  avia-builder-el-no-sibling  av-slideshow-ui av-control-hidden av-no-slider-navigation av-hide-nav-arrows av-slideshow-manual av-loop-once av-loop-manual-endless av-default-height-applied avia-slideshow-1' data-slideshow-options=\"{&quot;animation&quot;:&quot;fade&quot;,&quot;autoplay&quot;:false,&quot;loop_autoplay&quot;:&quot;once&quot;,&quot;interval&quot;:4,&quot;loop_manual&quot;:&quot;manual-endless&quot;,&quot;autoplay_stopper&quot;:false,&quot;noNavigation&quot;:true,&quot;bg_slider&quot;:false,&quot;keep_padding&quot;:false,&quot;hoverpause&quot;:false,&quot;show_slide_delay&quot;:0}\"  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><ul class='avia-slideshow-inner ' style='padding-bottom: 30.714285714286%;'><li  class='avia-slideshow-slide av-6crz4-8bc718b3dd7ccb72815dccf5412e448e__0  av-single-slide slide-1 slide-odd'><div data-rel='slideshow-1' class='avia-slide-wrap '   ><img fetchpriority=\"high\" class=\"wp-image-248 avia-img-lazy-loading-not-248\"  src='https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/04\/protocolsBanner.jpeg' width='840' height='258' title='protocolsBanner' alt=''  itemprop=\"thumbnailUrl\"   \/><\/div><\/li><\/ul><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-56hwo-b58d9ae950f8bb5beb55bc4176f073b1\">\n.flex_column.av-56hwo-b58d9ae950f8bb5beb55bc4176f073b1{\nborder-width:10px;\nborder-color:#ffffff;\nborder-style:solid;\nborder-radius:0px 0px 0px 0px;\npadding:0 0 0 10px;\nbackground-color:#cccccc;\n}\n<\/style>\n<div  class='flex_column av-56hwo-b58d9ae950f8bb5beb55bc4176f073b1 av_one_fourth  avia-builder-el-2  el_after_av_three_fourth  el_before_av_one_half  no_margin flex_column_table_cell av-equal-height-column av-align-top  '     ><div  class='avia-builder-widget-area clearfix  avia-builder-el-3  avia-builder-el-no-sibling '><div id=\"nav_menu-2\" class=\"widget clearfix widget_nav_menu\"><div class=\"menu-mainsideheadmenu-container\"><ul id=\"menu-mainsideheadmenu\" class=\"menu\"><li id=\"menu-item-47\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-home menu-item-47\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/\">HOME<\/a><\/li>\n<li id=\"menu-item-46\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-46\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/research\/\">RESEARCH<\/a><\/li>\n<li id=\"menu-item-45\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-45\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/publications\/\">PUBLICATIONS<\/a><\/li>\n<li id=\"menu-item-44\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-44\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/people\/\">PEOPLE<\/a><\/li>\n<li id=\"menu-item-43\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-43\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/protocols-reagents\/\">PROTOCOLS &#038; REAGENTS<\/a><\/li>\n<li id=\"menu-item-42\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-42\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/news-events\/\">NEWS &#038; EVENTS<\/a><\/li>\n<li id=\"menu-item-41\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-41\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/join-the-lab\/\">JOIN THE LAB<\/a><\/li>\n<li id=\"menu-item-40\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-40\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/contact-us\/\">CONTACT US<\/a><\/li>\n<li id=\"menu-item-381\" class=\"menu-item menu-item-type-custom menu-item-object-custom menu-item-381\"><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-vbtg\/\" title=\"vbtg\">VASCULAR BIOLOGY TRAINING GRANT<\/a><\/li>\n<\/ul><\/div><\/div><\/div><\/div><\/div><!--close column table wrapper. Autoclose: 1 -->\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-3ot6w-75bd448c5351ba2e21312b1b2d796338\">\n.flex_column.av-3ot6w-75bd448c5351ba2e21312b1b2d796338{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-3ot6w-75bd448c5351ba2e21312b1b2d796338 av_one_half  avia-builder-el-4  el_after_av_one_fourth  el_before_av_one_half  first flex_column_div av-zero-column-padding  column-top-margin'     ><section  class='av_textblock_section av-3g9xs-ecc385faf61415f07fba00f3ff832ea6 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_AA_Matrigel.pdf https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Antibodies-That-Work.xlsx\">Antibodies That Work<\/a><\/h4>\n<p><a class=\"mwm-aal-item\" name=\"transgenic-lines\"><\/a><\/p>\n<h4><\/h4>\n<h4>Transgenic lines:<\/h4>\n<p>Our laboratory has developed a constitutive VE Cre-Recombinase mouse line available to the scientific community through the Jax labs.<\/p>\n<p>Constitutive VE-Cadherin Cre-Recombinase<\/p>\n<ul>\n<li><a href=\"https:\/\/www.mcdb.ucla.edu\/Research\/Arispe\/?p=1681\">Publication characterizing the model:<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Constitutive_Vecad-cre-Prot.pdf\" rel=\"\">Protocol for genotyping<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Reagents_Transgenic_PubsCiting.pdf\">Resulting Pubs Using Transgenic Line<\/a><\/li>\n<\/ul>\n<p>Inducible VE-Cadherin Cre-Recombinase<\/p>\n<ul>\n<li><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.20982\/epdf\" target=\"_blank\" rel=\"noopener\">Publication characterizing the mode<\/a>l<a class=\"hiddennewsb\">:<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/JAX-Generic-Cre.pdf\" rel=\"\">Protocol for genotyping<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_animl_Tamoxifen-Prot.pdf\" target=\"_blank\" rel=\"noopener\">Protocols for tamoxifen-induction<\/a><\/li>\n<li><a class=\"hiddennewsb\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed?linkname=pubmed_pubmed_citedin&amp;from_uid=17072878\" target=\"_blank\" rel=\"noopener\">Resulting publications using this line<\/a><\/li>\n<\/ul>\n<p>Floxed progesterone receptor<\/p>\n<ul>\n<li><a class=\"hiddennewsb\" href=\"https:\/\/www.mcdb.ucla.edu\/Research\/Arispe\/?p=1693\">Publication characterizing the model:<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_PRCE-Genotyping.pdf\">PRCE Genotyping Protocol<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Reagents_FloxedProgest_PubsCiting.pdf\">Resulting Pubs Using Floxed Progest<\/a><\/li>\n<\/ul>\n<p>ADAMTS1 knock-out<\/p>\n<ul>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1636613\/\" target=\"_blank\" rel=\"noopener\">Publication characterizing the model<\/a><a class=\"hiddennewsb\">:<\/a><\/li>\n<li><a class=\"hiddennewsb\" href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/PCRgenotypingADAMTS1.pdf\">Protocol for genotyping<\/a><\/li>\n<li><a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Reagents_ADAMTS1_PubsCiting.pdf\">Resulting pubs using ADAMTS1<\/a><\/li>\n<\/ul>\n<\/div><\/section><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-2sc4s-aa85986c9589f1810072000b7c583c18\">\n.flex_column.av-2sc4s-aa85986c9589f1810072000b7c583c18{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-2sc4s-aa85986c9589f1810072000b7c583c18 av_one_half  avia-builder-el-6  el_after_av_one_half  el_before_av_one_full  flex_column_div av-zero-column-padding  column-top-margin'     ><section  class='av_textblock_section av-265tw-8030053fb3afe47fa8b4a9eb703036fd '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4 class=\"col-sm-12 col-md-6\"><strong>Protocols:<\/strong><\/h4>\n<div class=\"col-sm-12 col-md-6\">\n<ul>\n<li>Angiogenesis Assays \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_AA_Cam-Assay1.pdf\">Cam Assay<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_AA_Matrigel.pdf\">Matrigel<\/a><\/li>\n<li>Animal \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_XenograftTumorAssay.pdf\">XenograftTumorAssay<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_animl_Tamoxifen-Prot.pdf\">Tamoxifen Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_X-gal-StainingofCells.pdf\">X-gal Staining of Cells<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_Whole-mtISH_ChickEmbryo.pdf\">Whole-Mt ISH_Chicken Embryo<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_vascular-permeability.pdf\">Vascular Permeability<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_superovulation.pdf\">Superovulation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_Ovarian_Transplant.pdf\">Ovarian Transplant<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_Mouse_BoneMarrow_Transpl.pdf\">Mouse Bone Marrow Trans<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_MatrigelAssayMOD.pdf\">Matrigel Assay Modif.<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_FACSStain.pdf\">FACS Stain<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_EC_isolationMOD.pdf\">EC Isolation Modif.<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Animl_CutaneousWoundHealA.pdf\">Cutaneous Wound Healing<\/a><\/li>\n<li>Biochemistry \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_Zymogram.pdf\">Zymogram Protocol<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_VEGF_West_TricineGel.pdf\">VEGF West Tricine Gel<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_TricineGel_afterVEGFdigest.pdf\">Tricine Gel after VEGF digest<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_SilverStain.pdf\">Silver Staining<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_Membrane_Strip.pdf\">Membrane Stripping<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_membproteinelabel.pdf\">Membrane Protein labelling<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_LacZ_Stain.pdf\">LacZ Staining<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_Immunoprecipit.pdf\">Immunoprecipitation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Bchem_Biotinyl_VEGF_Digestion.pdf\">Biotinylated VEGF Digestion<\/a><\/li>\n<li>Genotyping \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_Ve-cad-cre-pcr.pdf\">Ve-cadherin Cre PCR<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_sexdetermination.pdf\">Sex Determination<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_ROSA_PCR_REACtion.pdf\">ROSA PCR REACtion<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_PCRgenotypADAMTS1KOmice.pdf\">PCR Genotype ADAMTS1 KO mice<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_PCR_PR_CRErecombinase.pdf\">PCR PR-CRE Recombinase<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_NOTCH_lox_PCR.pdf\">NOTCH lox PCR Prot.<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_MiceGenotypingLacZ.pdf\">Mice Genotyping by LacZ<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_ILK_PCR_REACtion.pdf\">ILK PCR REACtion<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_DNAGenotyping.pdf\">DNA Genotyping Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_CRE_PCR_REACtion.pdf\">CRE PCR REACtion<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_CIVE-cre-pcr.pdf\">CIVE PCR Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_BIF_pcr_REACtion.pdf\">BIF PCR REACtion<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Genotype_ADAMTS1_PCR.pdf\">ADAMTS1 KO mice PCR<\/a><\/li>\n<li>Immunohistochemistry \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_WholeMtRetinaIHC.pdf\">Whole Mt Retina IHC<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_VEcadherin.pdf\">VE-Cadherin Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_stainingMLC.pdf\">MLC for Cells in Suspension<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_QH1stain.pdf\">QH1 Antibody staining \u2013 Quail<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_PECAM.pdf\">PECAM Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_Mod_LacZstain.pdf\">Modified LacZ staining<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_MAB1398Z.pdf\">Hamster AntiMouse CD31<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_LacZstaining.pdf\">LacZ Staining \u2013 Mouse VR26<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_LacZstaining.pdf\">LacZ Staining Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_IHC_WholeMt_TissuePECAM.pdf\">IHC Whole Mt Tissue PECAM<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_IHC_of_CD312.pdf\">IHC \u2013 CD31 Paraffin Sections<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_FACSStaining.pdf\">FACS Staining Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_IHC_Antigen-retrieval.pdf\">Citrate Buffer Epitope<\/a><\/li>\n<li>Molecular Biology \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBiol_Blue_White_Color_Screening.pdf\">Blue White Color Screening<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBiol_Annealing_Adapters.pdf\">Annealing of Adapters<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_Whole_mt_ISH.pdf\">Whole Mt ISH Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_Vector_Insert_Ratio.pdf\">Ligations \u2013 Vector Insert Ratio<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_RNA_Protection_Assay.pdf\">RNA Protection Assay<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_RNA_Gel.pdf\">RNA Gel<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_RNA_Extraction.pdf\">RNA Extraction Solutions<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_RNA_Extraction_from_Cells.pdf\">RNA Extraction from Cells<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_Realtime-PCR.pdf\">Realtime PCR Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_Miniprep_DNA.pdf\">Miniprep DNA<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_MolBio_Genotyping_TGF-alpha.pdf\">Genotyping TGF-alpha Transgenic mice<\/a> <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/NanostringCounterSampleCollection-SOP.pdf\" rel=\"\">Nanostring Counter SOP<\/a><\/li>\n<li>Protein Chemistry \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_VitrogenGel.pdf\">3D Vitrogen Gel<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_VEGF_bound_plate.pdf\">Coating VEGF bound_plate<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_transfection.pdf\">Transfection of T47D Cells<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_SoftAgarColonyFormation.pdf\">Soft Agar Colony Formation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Isolation_OvarianEpithe.pdf\">Isolation of Ovarian Epithelial Cells<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_live_cells_stain.pdf\">Labeling cells with live dyes<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Isolation_OvarianEpithe.pdf\">Isolation of Ovarian Epithelial Cells<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Iodination_Inject.pdf\">Iodination Injection of 125I-Protein<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Immunoprecipit.pdf\">Immunoprecipitation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_HDEC_Isolation.pdf\">HDEC Isolation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_CV_StainingCells_CloneCounting.pdf\">CV cell staining &amp; clone counting<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Cell_Surface_Biotinylation.pdf\">Cell Surface Biotinylation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_BAEC_Isolation.pdf\">BAEC Isolation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_Adhesion_Assays.pdf\">Adhesion Assays<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_ProteinChem_ADAMTS1isolation.pdf\">ADAMTS1 Purification<\/a><\/li>\n<li>Subcloning Guide \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_EasySubcloning.pdf\">Easy Subcloning Prot<\/a><\/li>\n<li>Tissue Culture \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_X-galCell-Staining.pdf\">X-gal Staining \u2013 LacZ Expression<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_T-Production.pdf\">T-Prod. \u2013 Cell Line GgP+E<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_phosphorylation-assay.pdf\">KDR phosphoryl. assay<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_MediaRecipes.pdf\">Media Recipes<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_MAEC-isolation.pdf\">MAEC isolation<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_LentiviralInfectionofEndoCells.pdf\">Lentiviral Infection \u2013 HDMEC<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_IsolationEndoCells_MouseEmbryos.pdf\">Isolation of ECs from Mouse Embryos<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_Isolation-of-EC-from-embr.pdf\">Isolation of EC from embryo<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_IsolatingBAEC.pdf\">Isolating BAEC<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_Integrin_Binding_Assays.pdf\">Integrin Receptor Ligand Binding<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_IHC.pdf\">Immunocytochem<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_FACSstaining.pdf\">Staining Prot for FACS<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_CaPO4Transfection.pdf\">CaPO4 Transfection<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_TC_35S-methionLabeling.pdf\">35S-methionine Labeling<\/a><\/li>\n<li>Vascular Imaging \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_Whole-mt_Lectin_Stain.pdf\">Whole mt Lectin Stain w\/Vector Elite Kit<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_Whole-Mt-PECAM.pdf\">Whole Mt or Vibratome-Sect. Staining<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_Paraffin-PECAM.pdf\">Paraffin-Embedded PECAM<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_live_cell_stain.pdf\">Labeling Cells w\/ Live Dyes<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_ImmunostainCD31.pdf\">Immunostain for CD31<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_ImmunoperoxidaseOfPar.pdf\">Immunoperoxid of Paraffin Sect<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_ICC.pdf\">ICC Prot<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_VascImag_DABimmunostain_CD31.pdf\">DAB immunostain for CD31<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Prot_Vasc_Imag_WholeMt-Notch.pdf\">Whole Mt Notch Antibody Staining<\/a> \u2013 <a href=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/Vibratome-Protocol.pdf\">Vibratome Protocol<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div><\/section><\/div><\/p>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-jp0-8b31f3142addd700158bc81c6409283d\">\n.flex_column.av-jp0-8b31f3142addd700158bc81c6409283d{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\nbackground-color:#cccccc;\n}\n<\/style>\n<div  class='flex_column av-jp0-8b31f3142addd700158bc81c6409283d av_one_full  avia-builder-el-8  el_after_av_one_half  avia-builder-el-last  first flex_column_div av-zero-column-padding  column-top-margin'     ><div  class='avia-builder-widget-area clearfix  avia-builder-el-9  avia-builder-el-no-sibling '><div id=\"text-6\" class=\"widget clearfix widget_text\">\t\t\t<div class=\"textwidget\"><div style=\"background: #ccc; padding: 8px;height:71px !important; \"><a href=\"https:\/\/www.ucla.edu\" target=\"_blank\"><img decoding=\"async\" src=\"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-content\/uploads\/sites\/126\/2017\/05\/UCLA_logo_blue.png\" style=\"height:65px !important;float:right !important;\"><\/a><\/div><\/div>\n\t\t<\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-24","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/pages\/24","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/comments?post=24"}],"version-history":[{"count":9,"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/pages\/24\/revisions"}],"predecessor-version":[{"id":591,"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/pages\/24\/revisions\/591"}],"wp:attachment":[{"href":"https:\/\/sites.lifesci.ucla.edu\/mcdb-arispe\/wp-json\/wp\/v2\/media?parent=24"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}