Antibodies That Work
Transgenic lines:
Our laboratory has developed a constitutive VE Cre-Recombinase mouse line available to the scientific community through the Jax labs.
Constitutive VE-Cadherin Cre-Recombinase
Inducible VE-Cadherin Cre-Recombinase
- Publication characterizing the model:
- Protocol for genotyping
- Protocols for tamoxifen-induction
- Resulting publications using this line
Floxed progesterone receptor
ADAMTS1 knock-out
Protocols:
- Angiogenesis Assays – Cam Assay – Matrigel
- Animal – XenograftTumorAssay – Tamoxifen Prot – X-gal Staining of Cells – Whole-Mt ISH_Chicken Embryo – Vascular Permeability – Superovulation – Ovarian Transplant – Mouse Bone Marrow Trans – Matrigel Assay Modif. – FACS Stain – EC Isolation Modif. – Cutaneous Wound Healing
- Biochemistry – Zymogram Protocol – VEGF West Tricine Gel – Tricine Gel after VEGF digest – Silver Staining – Membrane Stripping – Membrane Protein labelling – LacZ Staining – Immunoprecipitation – Biotinylated VEGF Digestion
- Genotyping – Ve-cadherin Cre PCR – Sex Determination – ROSA PCR REACtion – PCR Genotype ADAMTS1 KO mice – PCR PR-CRE Recombinase – NOTCH lox PCR Prot. – Mice Genotyping by LacZ – ILK PCR REACtion – DNA Genotyping Prot – CRE PCR REACtion – CIVE PCR Prot – BIF PCR REACtion – ADAMTS1 KO mice PCR
- Immunohistochemistry – Whole Mt Retina IHC – VE-Cadherin Prot – MLC for Cells in Suspension – QH1 Antibody staining – Quail – PECAM Prot – Modified LacZ staining – Hamster AntiMouse CD31 – LacZ Staining – Mouse VR26 – LacZ Staining Prot – IHC Whole Mt Tissue PECAM – IHC – CD31 Paraffin Sections – FACS Staining Prot – Citrate Buffer Epitope
- Molecular Biology – Blue White Color Screening – Annealing of Adapters – Whole Mt ISH Prot – Ligations – Vector Insert Ratio – RNA Protection Assay – RNA Gel – RNA Extraction Solutions – RNA Extraction from Cells – Realtime PCR Prot – Miniprep DNA – Genotyping TGF-alpha Transgenic mice Nanostring Counter SOP
- Protein Chemistry – 3D Vitrogen Gel – Coating VEGF bound_plate – Transfection of T47D Cells – Soft Agar Colony Formation – Isolation of Ovarian Epithelial Cells – Labeling cells with live dyes – Isolation of Ovarian Epithelial Cells – Iodination Injection of 125I-Protein – Immunoprecipitation – HDEC Isolation – CV cell staining & clone counting – Cell Surface Biotinylation – BAEC Isolation – Adhesion Assays – ADAMTS1 Purification
- Subcloning Guide – Easy Subcloning Prot
- Tissue Culture – X-gal Staining – LacZ Expression – T-Prod. – Cell Line GgP+E – KDR phosphoryl. assay – Media Recipes – MAEC isolation – Lentiviral Infection – HDMEC – Isolation of ECs from Mouse Embryos – Isolation of EC from embryo – Isolating BAEC – Integrin Receptor Ligand Binding – Immunocytochem – Staining Prot for FACS – CaPO4 Transfection – 35S-methionine Labeling
- Vascular Imaging – Whole mt Lectin Stain w/Vector Elite Kit – Whole Mt or Vibratome-Sect. Staining – Paraffin-Embedded PECAM – Labeling Cells w/ Live Dyes – Immunostain for CD31 – Immunoperoxid of Paraffin Sect – ICC Prot – DAB immunostain for CD31 – Whole Mt Notch Antibody Staining – Vibratome Protocol
