• Publications

Toshima, J., Tsukahara, A., Nagano, M., Tojima, T., Siekhaus, D,E., Nakano, A., Toshima, J.
The yeast endocytic early/sorting compartment exists as an independent sub-compartment within the trans-Golgi network. (2023) Elife 12:e84850

Nagano, M., Aoshima, K., Shimamura, H., Siekhaus, D,E., Toshima, J.Y, Toshima, J., Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway. (2023) Journal of Cell Science 136(17):js261448.

Enshoji, M., Miyano, M., Yoshiada, N., Kunihiro, M., Nagano, M., Siekhaus D.E., Toshima, J.Y. and Toshima, J.
Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway. (2022) Journal of Cell Biology, 221(10):e.202112138

Akhmanova, M., Emtenani, S., Krueger, D., Gyoergy, A., Guarda, M., Vlasov, M., Vlasov, F., Akopian, A., Ratheesh, A., De Renzis, S., Siekhaus, D.E.
Cell division in tissues enables macrophage infiltration (2022) Science 376, 394-6.
https://www.science.org/doi/10.1126/science.abj0425
Video abstract: https://www.youtube.com/watch?v=zwoD6Ns-amc

Martin, E., Blatt, P., Pocchiari, T., Fuchs, G., Emtenani, S., Siekhaus, D.E., Rangan, P.,
A translational control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. (2022) Developmental Cell 57(7)883-900.e10
https://www.cell.com/developmental-cell/pdf/S1534-5807(22)00165-4.pdf

Emtenani, S., Martin, E., Gyoergy, A., Bicher, J., Genger, J.W., Hurd, T.R., Koecher, T., Berthaler, A. Rangan, P, Siekhaus, D.E.
Macrophage mitochondrial bioenergetics and tissue invasion are promoted by an Atossa-Porthos axis in Drosophila (2022) The EMBO Journal e109049
https://www.embopress.org/doi/full/10.15252/embj.2021109049
Video abstract: https://www.youtube.com/watch?v=hZRxBNjoU90

Roblek, M., Bicher, J., van Gogh, M., Gyoergy,  A., Seebock, R. Szulc, B.,  Damme, M., Olczak, M, Bursig, L., Siekhaus, D.E. 
The solute carrier MFSD1 decreases b1 Integrin’s activation status  and thus metastasis (2022) Frontiers in Oncology 12, 777634 
https://www.frontiersin.org/articles/10.3389/fonc.2022.777634/full

Belyaeva, V., Wachner, S., Gyoergy, A., Emtenani, S., Gridchyn, I., Akhmanova, M., Linder, M., Sibilia, M., Siekhaus, D.E.
Fos regulates macrophage infiltration against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila. (2022) PLOS Biology 20(1): e3001494
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001494
Video abstract:https://www.youtube.com/watch?v=bUfwz4n4zuk

Inglés-Prieto, A., Furthmann, N., Crossman, S.H., Tichy, A.M., Hoyer, N., Petersen, M., Zheden, V., Biebl, J., Reichart, E., György, A., Siekhaus, D.E., Soba, P., Winklhofer, K.F., Janovjak, H.
Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease. (2021) PLOS Genetics 17(4):e1009479

Kierdorf, K., Hersperger F., Sharrock, J.A., Vincent, C.M., Ustaoglu, P., Dou, J., Gyoergy, A., Gross, O., Siekhaus, D.E., Dionne, M.S.
Muscle function and homeostasis require macrophage-derived cytokine inhibition of AKT activity in Drosophila. (2020) Elife e51595
https://doi.org/10.7554/Elife.51595

Nagano, M., Toshima, J.Y., Siekhaus, D.E., Toshima, J.
Rab5-mediated endosome formation is regulated at the trans-Golgi network. (2019) Communications Biology 2:419

Valoskova, K., Biebl, J., Roblek, M., Emtenani , S., Gyoergy, A., Misova, M., Ratheesh, A., Shkarina, K., Larsen, I.S.B, Vakhrushev, S.Y., Clausen, H., Siekhaus, D.E. (2019)
A conserved MFS orchestrates a subset of O-glycosylation to facilitate macrophage dissemination and tissue invasion. Elife  e41801
https://doi.org/10.7554/eLife.41801

Trebuchet, G., Cattenoz, P., Zsamboki, J., Mazaud, D., Siekhaus, D.E., Fanto, M., and Giangrande, A. (2019)
The Repo homeodomain transcription factor suppresses hematopoiesis in Drosophila and preserves the glial fate. J. Neuroscience 39(2)238-255.

Ratheesh, A, Biebl, J., Vesela, J., Smutny, M., Papusheva, E. Krens, S.F.G, Kaufmann, W., Gyoergy, A., Casano, A.M., Siekhaus D.E. (2018)
Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell 45(3):331-346
https://www.cell.com/developmental-cell/fulltext/S1534-5807(18)30277-6

Gyoergy, A., Roblek, M., Ratheesh, A., Valoskova, K., Belyaeva, V., Wachner, S., Matsubayashi, Y., Sanchez-Sanchez, B.J., Stramer, B., and Siekhaus D.E. (2018)
Tools allowing independent visualization and genetic manipulation of Drosophila melanogaster macrophages and surrounding tissues. G3 (Bethesda) 8(3):845-857
https://academic.oup.com/g3journal/article/8/3/845/6027144
(The strains in this paper are available at Bloomington Stock Center)

Yamamoto, W., Wada, S., Nagano, M., Aoshima, K., Siekhaus, D.E., Toshima, J.Y., and Toshima, J. (2017)
Distinct roles for plasma membrane PtdIns(4)P and PtdIns(4,5)P2 during yeast receptor-mediated endocytosis. J. Cell Science 131(1) doi:10.1242/jcs.207696

Matsubayashi, Y., Louani, A., Dragu, A., Sánchez-Sánchez, B.J., Serna-Morales, E., Yolland, L., Gyoergy, A., L Vizcay, G., Fleck, R.A., Heddleston, J.M., Chew, T.L., Siekhaus D., Stramer B. (2017)
A moving source of matrix components is essential for de novo basement membrane formation. Current Biology 27(22):3526-34
https://doi.org/10.1016/j.cub.2017.10.001

Toshima, J.Y., Furuya, E., Nagano, M., Kanno, C., Sakamoto, Y., Ebihara, M., Siekhaus, D.E., Toshima, J. (2016)
Yeast Eps-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton. Elife e10276

Toshima, J.Y., Horikomi, C., Okada, A., Hatori M.N., Nagano, J., Masuda, A., Yamamoto, W., Siekhaus D.E., and Toshima, J. (2016)
Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis. J Cell Science 129(2):367-79

Ratheesh, A., Belyaeva, V., Siekhaus, D.E. (2015)
Drosophila immune cell migration and adhesion during embryonic development and larval immune responses.  Current Opin Cell Biol 36:71-7
https://doi.org/10.1016/j.ceb.2015.07.003

Kawada, D., Kobayashi, H., Tomita, T., Nakata E., Nagano, M., Siekhaus, D.E., Toshima, J.Y., Toshima, J. (2015)
The yeast ARF-GAP Glo3p is required for the endocytic recycling of cell surface proteins. Biochem Biophysica Acta 1853(1):144-156

Toshima, J.Y., Nishinoaki, S., Sato, Y., Yamamoto, W., Furukawa, D., Siekhaus D.E., Sawaguchi, A. and Toshima, J. (2014)
Bifurcation of the endocytic pathway into Rab5 dependent and independent transport to the vacuole. Nature Communications 5:3498

DeGennaro, M.*, Hurd, T.*, Siekhaus, D., Biteau, B., Jasper, H. and  R. Lehmann. (2011)
Peroxiredoxin stabilization of E-cadherin promotes primordial germ cell adhesion. Developmental Cell 20(2):233-243
*authors contributed equally

Siekhaus, D., Haesemeyer, M., Moffitt, O. and Lehmann, R. (2010)
RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. Nature Cell Biology 12(6):605-610

Kunwar, P.S., Siekhaus, D. E. and Lehmann, R. (2006)
In vivo migration: a germ cell perspective. Ann Rev of Cell and Develop Biology 22:237-65 

Siekhaus, D.E. and Drubin, D.G. (2003)
Spontaneous receptor-independent heterotrimeric G protein signaling in an RGS mutant. Nature Cell Biology 5(3):231-5

Rayburn, L.Y.M., Gooding, H.C. Choksi, S.P., Maloney, D., Kidd, A.R., Siekhaus, D.E. and Bender, M. (2003)
amontillado, the Drosophila homolog of the prohormone processing protease PC2, is required during embryogenesis and early larval development. Genetics 163(1):227-237

Hwang J.R., Siekhaus D.E., Fuller R.S., Taghert P.H. and Lindberg I. (2000)
Interaction of Drosophila melanogaster Prohormone Convertase 2 and 7B2. Insect Cell-Specific Processing and Secretion. J Biological Chemistry 275(23):17886-17893

Siekhaus, D.E. and Fuller, R.S. (1999)
A role for amontillado, the Drosophila homolog of the neuropeptide precursor processing protease PC2, in hatching behavior.  J Neurosci 19: 6942-6

Video Abstracts >