April Pyle* – Molecular Biology Institute

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Publications:

Hicks MR, Saleh KK, Clock B, Gibbs DE, Yang M, Younesi S, Gane L, Gutierrez-Garcia V, Xi H, Pyle AD. Publisher Correction: Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells. Nat Cell Biol. 2024 Feb; 26(2):306. view on PubMed

Romero MA, Pyle AD. 'Enhancing' skeletal muscle and stem cells in three-dimensions: genome regulation of skeletal muscle in development and disease. Curr Opin Genet Dev. 2023 12; 83:102133. view on PubMed

Hicks MR, Saleh KK, Clock B, Gibbs DE, Yang M, Younesi S, Gane L, Gutierrez-Garcia V, Xi H, Pyle AD. Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells. Nat Cell Biol. 2023 Dec; 25(12):1758-1773. view on PubMed

Hicks MR, Liu X, Young CS, Saleh K, Ji Y, Jiang J, Emami MR, Mokhonova E, Spencer MJ, Meng H, Pyle AD. Nanoparticles systemically biodistribute to regenerating skeletal muscle in DMD. J Nanobiotechnology. 2023 Aug 29; 21(1):303. view on PubMed

Jaime OG, Arias J, Pavani S, Pyle AD, Hicks MR. SIX1+PAX3+ identify a progenitor for myogenic lineage commitment from hPSCs. Development. 2023 07 15; 150(14). view on PubMed

Emami MR, Espinoza A, Young CS, Ma F, Farahat PK, Felgner PL, Chamberlain JS, Xu X, Pyle AD, Pellegrini M, Villalta SA, Spencer MJ. Innate and adaptive AAV-mediated immune responses in a mouse model of Duchenne muscular dystrophy. Mol Ther Methods Clin Dev. 2023 Sep 14; 30:90-102. view on PubMed

Gibbs DE, Pyle AD. Muscle fusogens go viral for gene delivery to skeletal muscle. Cell. 2023 05 11; 186(10):2041-2043. view on PubMed

Saleh KK, Switzler C, Hicks MR, Gane L, Gibbs DE, Pyle AD. Duchenne muscular dystrophy disease severity impacts skeletal muscle progenitor cells systemic delivery. Front Physiol. 2023; 14:1190524. view on PubMed

Stearns-Reider KM, Hicks MR, Hammond KG, Reynolds JC, Maity A, Kurmangaliyev YZ, Chin J, Stieg AZ, Geisse NA, Hohlbauch S, Kaemmer S, Schmitt LR, Pham TT, Yamauchi K, Novitch BG, Wollman R, Hansen KC, Pyle AD, Crosbie RH. Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis. NPJ Regen Med. 2023 Mar 15; 8(1):16. view on PubMed

Saleh KK, Xi H, Switzler C, Skuratovsky E, Romero MA, Chien P, Gibbs D, Gane L, Hicks MR, Spencer MJ, Pyle AD. Single cell sequencing maps skeletal muscle cellular diversity as disease severity increases in dystrophic mouse models. iScience. 2022 Nov 18; 25(11):105415. view on PubMed

Wei Y, Qin Q, Yan C, Hayes MN, Garcia SP, Xi H, Do D, Jin AH, Eng TC, McCarthy KM, Adhikari A, Onozato ML, Spentzos D, Neilsen GP, Iafrate AJ, Wexler LH, Pyle AD, Suvà ML, Dela Cruz F, Pinello L, Langenau DM. Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcoma. Nat Cancer. 2022 08; 3(8):961-975. view on PubMed

Hicks MR, Pyle AD. The emergence of the stem cell niche. Trends Cell Biol. 2023 02; 33(2):112-123. view on PubMed

Chien P, Xi H, Pyle AD. Recapitulating human myogenesis ex vivo using human pluripotent stem cells. Exp Cell Res. 2022 02 15; 411(2):112990. view on PubMed

Nitzahn M, Truong B, Khoja S, Vega-Crespo A, Le C, Eliav A, Makris G, Pyle AD, Häberle J, Lipshutz GS. CRISPR-Mediated Genomic Addition to CPS1 Deficient iPSCs is Insufficient to Restore Nitrogen Homeostasis. Yale J Biol Med. 2021 12; 94(4):545-557. view on PubMed

Petrigliano FA, Liu NQ, Lee S, Tassey J, Sarkar A, Lin Y, Li L, Yu Y, Geng D, Zhang J, Shkhyan R, Bogdanov J, Van Handel B, Ferguson GB, Lee Y, Hinderer S, Tseng KC, Kavanaugh A, Crump JG, Pyle AD, Schenke-Layland K, Billi F, Wang L, Lieberman J, Hurtig M, Evseenko D. Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. NPJ Regen Med. 2021 Nov 23; 6(1):77. view on PubMed

Harn HI, Wang SP, Lai YC, Van Handel B, Liang YC, Tsai S, Schiessl IM, Sarkar A, Xi H, Hughes M, Kaemmer S, Tang MJ, Peti-Peterdi J, Pyle AD, Woolley TE, Evseenko D, Jiang TX, Chuong CM. Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. Nat Commun. 2021 05 10; 12(1):2595. view on PubMed

Xi H, Young CS, Pyle AD. Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells. STAR Protoc. 2020 Dec 18; 1(3):100158. view on PubMed

Liu J, Campagna J, John V, Damoiseaux R, Mokhonova E, Becerra D, Meng H, McNally EM, Pyle AD, Kramerova I, Spencer MJ. A Small-Molecule Approach to Restore a Slow-Oxidative Phenotype and Defective CaMKIIβ Signaling in Limb Girdle Muscular Dystrophy. Cell Rep Med. 2020 10 20; 1(7):100122. view on PubMed

Sengupta K, Mishra MK, Loro E, Spencer MJ, Pyle AD, Khurana TS. Genome Editing-Mediated Utrophin Upregulation in Duchenne Muscular Dystrophy Stem Cells. Mol Ther Nucleic Acids. 2020 Dec 04; 22:500-509. view on PubMed

Xi H, Langerman J, Sabri S, Chien P, Young CS, Younesi S, Hicks M, Gonzalez K, Fujiwara W, Marzi J, Liebscher S, Spencer M, Van Handel B, Evseenko D, Schenke-Layland K, Plath K, Pyle AD. A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Cell Stem Cell. 2020 Jul 02; 27(1):181-185. view on PubMed

Mosich GM, Husman R, Shah P, Sharma A, Rezzadeh K, Aderibigbe T, Hu VJ, McClintick DJ, Wu G, Gatto JD, Xi H, Pyle AD, Péault B, Petrigliano FA, Dar A. Non-fibro-adipogenic pericytes from human embryonic stem cells attenuate degeneration of the chronically injured mouse muscle. JCI Insight. 2019 12 19; 4(24). view on PubMed

Xi H, Pyle A. Beyond the genome: RNA control of stem cells. Science. 2019 11 08; 366(6466):684-685. view on PubMed

Bellmann J, Goswami RY, Girardo S, Rein N, Hosseinzadeh Z, Hicks MR, Busskamp V, Pyle AD, Werner C, Sterneckert J. A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits. Biomaterials. 2019 12; 225:119537. view on PubMed

Young CS, Pyle AD, Spencer MJ. CRISPR for Neuromuscular Disorders: Gene Editing and Beyond. Physiology (Bethesda). 2019 09 01; 34(5):341-353. view on PubMed

Hazim RA, Karumbayaram S, Jiang M, Dimashkie A, Lopes VS, Li D, Burgess BL, Vijayaraj P, Alva-Ornelas JA, Zack JA, Kohn DB, Gomperts BN, Pyle AD, Lowry WE, Williams DS. Correction to: Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2019 Feb 12; 10(1):52. view on PubMed

Ferguson GB, Van Handel B, Bay M, Fiziev P, Org T, Lee S, Shkhyan R, Banks NW, Scheinberg M, Wu L, Saitta B, Elphingstone J, Larson AN, Riester SM, Pyle AD, Bernthal NM, Mikkola HK, Ernst J, van Wijnen AJ, Bonaguidi M, Evseenko D. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nat Commun. 2018 09 07; 9(1):3634. view on PubMed

Roberts DC, Taylor ME, Pyle AD. Suicide Prevention for School Communities: An Educational Initiative for Student Safety. NASN Sch Nurse. 2018 May; 33(3):168-176. view on PubMed

Hicks MR, Hiserodt J, Paras K, Fujiwara W, Eskin A, Jan M, Xi H, Young CS, Evseenko D, Nelson SF, Spencer MJ, Handel BV, Pyle AD. ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs. Nat Cell Biol. 2018 01; 20(1):46-57. view on PubMed

Hazim RA, Karumbayaram S, Jiang M, Dimashkie A, Lopes VS, Li D, Burgess BL, Vijayaraj P, Alva-Ornelas JA, Zack JA, Kohn DB, Gomperts BN, Pyle AD, Lowry WE, Williams DS. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017 10 02; 8(1):217. view on PubMed

Xi H, Fujiwara W, Gonzalez K, Jan M, Liebscher S, Van Handel B, Schenke-Layland K, Pyle AD. In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Cell Rep. 2017 02 07; 18(6):1573-1585. view on PubMed

Young CS, Mokhonova E, Quinonez M, Pyle AD, Spencer MJ. Creation of a Novel Humanized Dystrophic Mouse Model of Duchenne Muscular Dystrophy and Application of a CRISPR/Cas9 Gene Editing Therapy. J Neuromuscul Dis. 2017; 4(2):139-145. view on PubMed

Lee PC, Truong B, Vega-Crespo A, Gilmore WB, Hermann K, Angarita SA, Tang JK, Chang KM, Wininger AE, Lam AK, Schoenberg BE, Cederbaum SD, Pyle AD, Byrne JA, Lipshutz GS. Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells. Mol Ther Nucleic Acids. 2016 Nov 29; 5(11):e394. view on PubMed

Young CS, Pyle AD. Exon Skipping Therapy. Cell. 2016 Nov 17; 167(5):1144. view on PubMed

Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ, Pyle AD. A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells. Cell Stem Cell. 2016 Apr 07; 18(4):533-40. view on PubMed

Thakore-Shah K, Koleilat T, Jan M, John A, Pyle AD. REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells. PLoS One. 2015; 10(12):e0145280. view on PubMed

Hicks M, Pyle A. The Path from Pluripotency to Skeletal Muscle: Developmental Myogenesis Guides the Way. Cell Stem Cell. 2015 Sep 03; 17(3):255-7. view on PubMed

Hou S, Choi JS, Chen KJ, Zhang Y, Peng J, Garcia MA, Yu JH, Thakore-Shah K, Ro T, Chen JF, Peyda P, Fan G, Pyle AD, Wang H, Tseng HR. Supramolecular nanosubstrate-mediated delivery for reprogramming and transdifferentiation of mammalian cells. Small. 2015 Jun 03; 11(21):2499-504. view on PubMed

Peng J, Garcia MA, Choi JS, Zhao L, Chen KJ, Bernstein JR, Peyda P, Hsiao YS, Liu KW, Lin WY, Pyle AD, Wang H, Hou S, Tseng HR. Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency. ACS Nano. 2014 May 27; 8(5):4621-9. view on PubMed

Awe JP, Lee PC, Ramathal C, Vega-Crespo A, Durruthy-Durruthy J, Cooper A, Karumbayaram S, Lowry WE, Clark AT, Zack JA, Sebastiano V, Kohn DB, Pyle AD, Martin MG, Lipshutz GS, Phelps PE, Pera RA, Byrne JA. Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status. Stem Cell Res Ther. 2013 Jul 26; 4(4):87. view on PubMed

Sherman SP, Pyle AD. Small molecule screening with laser cytometry can be used to identify pro-survival molecules in human embryonic stem cells. PLoS One. 2013; 8(1):e54948. view on PubMed

Tran T, Andersen R, Sherman SP, Pyle AD. Insights into skeletal muscle development and applications in regenerative medicine. Int Rev Cell Mol Biol. 2013; 300:51-83. view on PubMed

Palomares K, Vigant F, Van Handel B, Pernet O, Chikere K, Hong P, Sherman SP, Patterson M, An DS, Lowry WE, Mikkola HK, Morizono K, Pyle AD, Lee B. Nipah virus envelope-pseudotyped lentiviruses efficiently target ephrinB2-positive stem cell populations in vitro and bypass the liver sink when administered in vivo. J Virol. 2013 Feb; 87(4):2094-108. view on PubMed

Yanes RE, Tarn D, Hwang AA, Ferris DP, Sherman SP, Thomas CR, Lu J, Pyle AD, Zink JI, Tamanoi F. Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition. Small. 2013 Mar 11; 9(5):697-704. view on PubMed

Jonas SJ, Alva JA, Richardson W, Sherman SP, Galic Z, Pyle AD, Dunn B. A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells. Mater Sci Eng C Mater Biol Appl. 2013 Jan 01; 33(1):234-41. view on PubMed

Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE. From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols. Stem Cells Transl Med. 2012 Jan; 1(1):36-43. view on PubMed

Alva JA, Lee GE, Escobar EE, Pyle AD. Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells. Stem Cells. 2011 Dec; 29(12):1952-62. view on PubMed

Le Belle JE, Orozco NM, Paucar AA, Saxe JP, Mottahedeh J, Pyle AD, Wu H, Kornblum HI. Proliferative neural stem cells have high endogenous ROS levels that regulate self-renewal and neurogenesis in a PI3K/Akt-dependant manner. Cell Stem Cell. 2011 Jan 07; 8(1):59-71. view on PubMed

Sherman SP, Alva JA, Thakore-Shah K, Pyle AD. Human pluripotent stem cells: the development of high-content screening strategies. Methods Mol Biol. 2011; 767:283-95. view on PubMed

Tchieu J, Kuoy E, Chin MH, Trinh H, Patterson M, Sherman SP, Aimiuwu O, Lindgren A, Hakimian S, Zack JA, Clark AT, Pyle AD, Lowry WE, Plath K. Female human iPSCs retain an inactive X chromosome. Cell Stem Cell. 2010 Sep 03; 7(3):329-42. view on PubMed

Tseng HC, Arasteh A, Paranjpe A, Teruel A, Yang W, Behel A, Alva JA, Walter G, Head C, Ishikawa TO, Herschman HR, Cacalano N, Pyle AD, Park NH, Jewett A. Increased lysis of stem cells but not their differentiated cells by natural killer cells; de-differentiation or reprogramming activates NK cells. PLoS One. 2010 Jul 16; 5(7):e11590. view on PubMed

Kamei K, Ohashi M, Gschweng E, Ho Q, Suh J, Tang J, For Yu ZT, Clark AT, Pyle AD, Teitell MA, Lee KB, Witte ON, Tseng HR. Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions. Lab Chip. 2010 May 07; 10(9):1113-9. view on PubMed

Zeng W, de Greef JC, Chen YY, Chien R, Kong X, Gregson HC, Winokur ST, Pyle A, Robertson KD, Schmiesing JA, Kimonis VE, Balog J, Frants RR, Ball AR, Lock LF, Donovan PJ, van der Maarel SM, Yokomori K. Specific loss of histone H3 lysine 9 trimethylation and HP1gamma/cohesin binding at D4Z4 repeats is associated with facioscapulohumeral dystrophy (FSHD). PLoS Genet. 2009 Jul; 5(7):e1000559. view on PubMed

Chin MH, Mason MJ, Xie W, Volinia S, Singer M, Peterson C, Ambartsumyan G, Aimiuwu O, Richter L, Zhang J, Khvorostov I, Ott V, Grunstein M, Lavon N, Benvenisty N, Croce CM, Clark AT, Baxter T, Pyle AD, Teitell MA, Pelegrini M, Plath K, Lowry WE. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell. 2009 Jul 02; 5(1):111-23. view on PubMed

Damoiseaux R, Sherman SP, Alva JA, Peterson C, Pyle AD. Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells. Stem Cells. 2009 Mar; 27(3):533-542. view on PubMed

Conway AE, Lindgren A, Galic Z, Pyle AD, Wu H, Zack JA, Pelligrini M, Teitell MA, Clark AT. A self-renewal program controls the expansion of genetically unstable cancer stem cells in pluripotent stem cell-derived tumors. Stem Cells. 2009 Jan; 27(1):18-28. view on PubMed

Wu H, Kim KJ, Mehta K, Paxia S, Sundstrom A, Anantharaman T, Kuraishy AI, Doan T, Ghosh J, Pyle AD, Clark A, Lowry W, Fan G, Baxter T, Mishra B, Sun Y, Teitell MA. Copy number variant analysis of human embryonic stem cells. Stem Cells. 2008 Jun; 26(6):1484-9. view on PubMed

Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells. 2008 Jun; 26(6):1436-43. view on PubMed

Lowry WE, Richter L, Yachechko R, Pyle AD, Tchieu J, Sridharan R, Clark AT, Plath K. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26; 105(8):2883-8. view on PubMed

Pyle AD, Lock LF, Donovan PJ. Neurotrophins mediate human embryonic stem cell survival. Nat Biotechnol. 2006 Mar; 24(3):344-50. view on PubMed

Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens. Methods Mol Biol. 2006; 325:207-24. view on PubMed

Dravid G, Ye Z, Hammond H, Chen G, Pyle A, Donovan P, Yu X, Cheng L. Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells. Stem Cells. 2005 Nov-Dec; 23(10):1489-501. view on PubMed

Pyle AD, Donovan PJ, Lock LF. Chipping away at 'stemness'. Genome Biol. 2004; 5(8):235. view on PubMed

Sharan SK, Pyle A, Coppola V, Babus J, Swaminathan S, Benedict J, Swing D, Martin BK, Tessarollo L, Evans JP, Flaws JA, Handel MA. BRCA2 deficiency in mice leads to meiotic impairment and infertility. Development. 2004 Jan; 131(1):131-42. view on PubMed

Pyle A, Handel MA. Meiosis in male PL/J mice: a genetic model for gametic aneuploidy. Mol Reprod Dev. 2003 Apr; 64(4):471-81. view on PubMed

Eaker S, Cobb J, Pyle A, Handel MA. Meiotic prophase abnormalities and metaphase cell death in MLH1-deficient mouse spermatocytes: insights into regulation of spermatogenic progress. Dev Biol. 2002 Sep 01; 249(1):85-95. view on PubMed

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