James Wohlschlegel – Molecular Biology Institute

Publications:

Blackwell AM, Jami-Alahmadi Y, Nasamu AS, Kudo S, Senoo A, Slam C, Tsumoto K, Wohlschlegel JA, Caaveiro JMM, Goldberg DE, Sigala PA. Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis. bioRxiv. 2024 Oct 13. view on PubMed

Soto JS, Neupane C, Kaur M, Pandey V, Wohlschlegel JA, Khakh BS. Astrocyte Gi-GPCR signaling corrects compulsive-like grooming and anxiety-related behaviors in Sapap3 knockout mice. Neuron. 2024 Oct 23; 112(20):3412-3423.e6. view on PubMed

Schuman Z, Xie Y, O'Keeffe S, Guan X, Sha J, Sun J, Wohlschlegel JA, Park JO, Liu C. Integrated Proteomics and Metabolomics Reveal Altered Metabolic Regulation of Xanthobacter autotrophicus under Electrochemical Water-Splitting Conditions. ACS Appl Mater Interfaces. 2024 Aug 07; 16(31):40973-40979. view on PubMed

Godoy MI, Pandey V, Wohlschlegel JA, Zhang Y. Secretome analysis of oligodendrocytes and precursors reveals their roles as contributors to the extracellular matrix and potential regulators of inflammation. bioRxiv. 2024 Jul 23. view on PubMed

Pasquarelli RR, Sha J, Wohlschlegel JA, Bradley PJ. BCC0 collaborates with IMC32 and IMC43 to form the Toxoplasma gondii essential daughter bud assembly complex. PLoS Pathog. 2024 Jul; 20(7):e1012411. view on PubMed

Geroyska S, Mejia I, Chan AA, Navarrete M, Pandey V, Kharpatin S, Noguti J, Wang F, Srole D, Chou TF, Wohlschlegel J, Nemeth E, Damoiseaux R, Shackelford DB, Lee DJ, Díaz B. N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma. Cancer Res Commun. 2024 Jul 01; 4(7):1815-1833. view on PubMed

Aguadé-Gorgorió J, Jami-Alahmadi Y, Calvanese V, Kardouh M, Fares I, Johnson H, Rezek V, Ma F, Magnusson M, Wang Y, Shin JE, Nance KJ, Goodridge HS, Liebscher S, Schenke-Layland K, Crooks GM, Wohlschlegel JA, Mikkola HKA. MYCT1 controls environmental sensing in human haematopoietic stem cells. Nature. 2024 Jun; 630(8016):412-420. view on PubMed

Shen JZ, Qiu Z, Wu Q, Zhang G, Harris R, Sun D, Rantala J, Barshop WD, Zhao L, Lv D, Won KA, Wohlschlegel J, Sangfelt O, Laman H, Rich JN, Spruck C. Retraction Notice to: A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell. 2024 06 20; 84(12):2398. view on PubMed

Pasquarelli RR, Quan JJ, Cheng ES, Yang V, Britton TA, Sha J, Wohlschlegel JA, Bradley PJ. Characterization and functional analysis of Toxoplasma Golgi-associated proteins identified by proximity labelling. bioRxiv. 2024 May 22. view on PubMed

Quan JJ, Nikolov LA, Sha J, Wohlschlegel JA, Coppens I, Bradley PJ. Systematic characterization of all Toxoplasma gondii TBC domain-containing proteins identifies an essential regulator of Rab2 in the secretory pathway. PLoS Biol. 2024 May; 22(5):e3002634. view on PubMed

Damianov A, Lin CH, Huang J, Zhou L, Jami-Alahmadi Y, Peyda P, Wohlschlegel J, Black DL. The splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. Mol Cell. 2024 Apr 18; 84(8):1496-1511.e7. view on PubMed

Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS. Crym-positive striatal astrocytes gate perseverative behaviour. Nature. 2024 Mar; 627(8003):358-366. view on PubMed

Fierro MA, Muheljic A, Sha J, Wohlschlegel J, Beck JR. PEXEL is a proteolytic maturation site for both exported and non-exported Plasmodium proteins. mSphere. 2024 Feb 28; 9(2):e0039323. view on PubMed

Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, Wohlschlegel JA, Aso Y, Zipursky SL. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. 2024 Mar 20; 112(6):942-958.e13. view on PubMed

Felgines L, Rymen B, Martins LM, Xu G, Matteoli C, Himber C, Zhou M, Eis J, Coruh C, Böhrer M, Kuhn L, Chicher J, Pandey V, Hammann P, Wohlschlegel J, Waltz F, Law JA, Blevins T. CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing. bioRxiv. 2023 Dec 26. view on PubMed

Soto JS, Jami-Alahmadi Y, Wohlschlegel JA, Khakh BS. In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation. Nat Protoc. 2024 Mar; 19(3):896-927. view on PubMed

Ta L, Tsai BL, Deng W, Sha J, Varuzhanyan G, Tran W, Wohlschlegel JA, Carr-Ascher JR, Witte ON. Wild-type C-Raf gene dosage and dimerization drive prostate cancer metastasis. iScience. 2023 Dec 15; 26(12):108480. view on PubMed

Boone BA, Ichino L, Wang S, Gardiner J, Yun J, Jami-Alahmadi Y, Sha J, Mendoza CP, Steelman BJ, van Aardenne A, Kira-Lucas S, Trentchev I, Wohlschlegel JA, Jacobsen SE. ACD15, ACD21, and SLN regulate the accumulation and mobility of MBD6 to silence genes and transposable elements. Sci Adv. 2023 11 17; 9(46):eadi9036. view on PubMed

Zemke NR, Hsu E, Barshop WD, Sha J, Wohlschlegel JA, Berk AJ. Adenovirus E1A binding to DCAF10 targets proteasomal degradation of RUVBL1/2 AAA+ ATPases required for quaternary assembly of multiprotein machines, innate immunity, and responses to metabolic stress. J Virol. 2023 Dec 21; 97(12):e0099323. view on PubMed

Xie Y, Ersan S, Guan X, Wang J, Sha J, Xu S, Wohlschlegel JA, Park JO, Liu C. Unexpected metabolic rewiring of CO2 fixation in H2-mediated materials-biology hybrids. Proc Natl Acad Sci U S A. 2023 10 17; 120(42):e2308373120. view on PubMed

Wong M, Martinez T, Tran M, Zuvia C, Gadkari A, Omaiye EE, Luo W, McWhirter KJ, Sha J, Kassem A, Wohlschlegel J, Talbot P. A synthetic coolant (WS-23) in disposable electronic cigarettes impairs cytoskeletal function in EpiAirway microtissues exposed at the air liquid interface. Sci Rep. 2023 10 07; 13(1):16906. view on PubMed

Boone BA, Ichino L, Wang S, Gardiner J, Yun J, Jami-Alahmadi Y, Sha J, Mendoza CP, Steelman BJ, van Aardenne A, Kira-Lucas S, Trentchev I, Wohlschlegel JA, Jacobsen SE. ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements. bioRxiv. 2023 Oct 06. view on PubMed

Pasquarelli RR, Back PS, Sha J, Wohlschlegel JA, Bradley PJ. Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii. PLoS Pathog. 2023 Oct; 19(10):e1011707. view on PubMed

Thind AC, Mota CM, Gonçalves APN, Sha J, Wohlschlegel JA, Mineo TWP, Bradley PJ. The Toxoplasma gondii effector GRA83 modulates the host's innate immune response to regulate parasite infection. mSphere. 2023 10 24; 8(5):e0026323. view on PubMed

Damianov A, Lin CH, Huang J, Zhou L, Jami-Alahmadi Y, Wohlschlegel J, Black DL. The apoptotic splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. bioRxiv. 2023 Sep 21. view on PubMed

Shimogawa MM, Wijono AS, Wang H, Zhang J, Sha J, Szombathy N, Vadakkan S, Pelayo P, Jonnalagadda K, Wohlschlegel J, Zhou ZH, Hill KL. FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction. Nat Commun. 2023 08 26; 14(1):5225. view on PubMed

Brunner A, Li Q, Fisicaro S, Kourtesakis A, Viiliäinen J, Johansson HJ, Pandey V, Mayank AK, Lehtiö J, Wohlschlegel JA, Spruck C, Rantala JK, Orre LM, Sangfelt O. FBXL12 degrades FANCD2 to regulate replication recovery and promote cancer cell survival under conditions of replication stress. Mol Cell. 2023 10 19; 83(20):3720-3739.e8. view on PubMed

Fierro MA, Muheljic A, Sha J, Wohlschlegel JA, Beck JR. PEXEL is a proteolytic maturation site for both exported and non-exported Plasmodium proteins. bioRxiv. 2023 Jul 12. view on PubMed

Li Z, Wang M, Zhong Z, Gallego-Bartolomé J, Feng S, Jami-Alahmadi Y, Wang X, Wohlschlegel J, Bischof S, Long JA, Jacobsen SE. The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation. Nat Commun. 2023 07 12; 14(1):4135. view on PubMed

Yao Z, Ramachandran S, Huang S, Jami-Alahmadi Y, Wohlschlegel JA, Li MMH. Chikungunya virus glycoproteins transform macrophages into productive viral dissemination vessels. bioRxiv. 2023 May 29. view on PubMed

Quan JJ, Nikolov LA, Sha J, Wohlschlegel JA, Bradley PJ. Toxoplasma gondii encodes an array of TBC-domain containing proteins including an essential regulator that targets Rab2 in the secretory pathway. bioRxiv. 2023 May 29. view on PubMed

Salas N, Blasco Pedreros M, Dos Santos Melo T, Maguire VG, Sha J, Wohlschlegel JA, Pereira-Neves A, de Miguel N. Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication. Elife. 2023 May 02; 12. view on PubMed

Espino-Sanchez TJ, Wienkers H, Marvin RG, Nalder SA, García-Guerrero AE, VanNatta PE, Jami-Alahmadi Y, Mixon Blackwell A, Whitby FG, Wohlschlegel JA, Kieber-Emmons MT, Hill CP, Sigala PA. Direct tests of cytochrome c and c1 functions in the electron transport chain of malaria parasites. Proc Natl Acad Sci U S A. 2023 05 09; 120(19):e2301047120. view on PubMed

Zhong Z, Xue Y, Harris CJ, Wang M, Li Z, Ke Y, Liu M, Zhou J, Jami-Alahmadi Y, Feng S, Wohlschlegel JA, Jacobsen SE. MORC proteins regulate transcription factor binding by mediating chromatin compaction in active chromatin regions. Genome Biol. 2023 04 26; 24(1):96. view on PubMed

Soto JS, Jami-Alahmadi Y, Chacon J, Moye SL, Diaz-Castro B, Wohlschlegel JA, Khakh BS. Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms. Nature. 2023 04; 616(7958):764-773. view on PubMed

Wang M, Zhong Z, Gallego-Bartolomé J, Feng S, Shih YH, Liu M, Zhou J, Richey JC, Ng C, Jami-Alahmadi Y, Wohlschlegel J, Wu K, Jacobsen SE. Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14. Nat Commun. 2023 03 28; 14(1):1736. view on PubMed

Afshar Y, Ma F, Quach A, Jeong A, Sunshine HL, Freitas V, Jami-Alahmadi Y, Helaers R, Li X, Pellegrini M, Wohlschlegel JA, Romanoski CE, Vikkula M, Iruela-Arispe ML. Transcriptional drifts associated with environmental changes in endothelial cells. Elife. 2023 03 27; 12. view on PubMed

Wang M, Zhong Z, Gallego-Bartolomé J, Li Z, Feng S, Kuo HY, Kan RL, Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, Wohlschlegel J, Jacobsen SE. A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis. Nat Plants. 2023 03; 9(3):460-472. view on PubMed

Obi CD, Dailey HA, Jami-Alahmadi Y, Wohlschlegel JA, Medlock AE. Proteomic Analysis of Ferrochelatase Interactome in Erythroid and Non-Erythroid Cells. Life (Basel). 2023 Feb 18; 13(2). view on PubMed

Espino-Sanchez TJ, Wienkers H, Marvin RG, Nalder SA, García-Guerrero AE, VanNatta PE, Jami-Alahmadi Y, Blackwell AM, Whitby FG, Wohlschlegel JA, Kieber-Emmons MT, Hill CP, Sigala PA. Direct Tests of Cytochrome Function in the Electron Transport Chain of Malaria Parasites. bioRxiv. 2023 Jan 23. view on PubMed

Back PS, Moon AS, Pasquarelli RR, Bell HN, Torres JA, Chen AL, Sha J, Vashisht AA, Wohlschlegel JA, Bradley PJ. IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome. mBio. 2023 02 28; 14(1):e0304222. view on PubMed

Gangwani MR, Soto JS, Jami-Alahmadi Y, Tiwari S, Kawaguchi R, Wohlschlegel JA, Khakh BS. Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors. Cell Rep. 2023 01 31; 42(1):111953. view on PubMed

Pandey V, Mayank AK, Wohlschlegel JA. Analyzing Iron and Oxygen-Regulated Protein Complex Formation Using Proteomic Mass Spectrometry. Methods Mol Biol. 2023; 2648:155-166. view on PubMed

Qian K, Tol MJ, Wu J, Uchiyama LF, Xiao X, Cui L, Bedard AH, Weston TA, Rajendran PS, Vergnes L, Shimanaka Y, Yin Y, Jami-Alahmadi Y, Cohn W, Bajar BT, Lin CH, Jin B, DeNardo LA, Black DL, Whitelegge JP, Wohlschlegel JA, Reue K, Shivkumar K, Chen FJ, Young SG, Li P, Tontonoz P. CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization. Nature. 2023 01; 613(7942):160-168. view on PubMed

Zhong Z, Wang Y, Wang M, Yang F, Thomas QA, Xue Y, Zhang Y, Liu W, Jami-Alahmadi Y, Xu L, Feng S, Marquardt S, Wohlschlegel JA, Ausin I, Jacobsen SE. Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis. Nat Commun. 2022 11 15; 13(1):6970. view on PubMed

Oravcová M, Nie M, Zilio N, Maeda S, Jami-Alahmadi Y, Lazzerini-Denchi E, Wohlschlegel JA, Ulrich HD, Otomo T, Boddy MN. The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers. Elife. 2022 11 14; 11. view on PubMed

Deng W, Sha J, Xue F, Jami-Alahmadi Y, Plath K, Wohlschlegel J. High-Field Asymmetric Waveform Ion Mobility Spectrometry Interface Enhances Parallel Reaction Monitoring on an Orbitrap Mass Spectrometer. Anal Chem. 2022 11 22; 94(46):15939-15947. view on PubMed

Diego-Martin B, Pérez-Alemany J, Candela-Ferre J, Corbalán-Acedo A, Pereyra J, Alabadí D, Jami-Alahmadi Y, Wohlschlegel J, Gallego-Bartolomé J. The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and transcription start site determination in Arabidopsis. Nucleic Acids Res. 2022 10 14; 50(18):10399-10417. view on PubMed

Yang E, Huang S, Jami-Alahmadi Y, McInerney GM, Wohlschlegel JA, Li MMH. Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes. PLoS Pathog. 2022 09; 18(9):e1010743. view on PubMed

Yang R, Meyer AS, Droujinine IA, Udeshi ND, Hu Y, Guo J, McMahon JA, Carey DK, Xu C, Fang Q, Sha J, Qin S, Rocco D, Wohlschlegel J, Ting AY, Carr SA, Perrimon N, McMahon AP. A genetic model for in vivo proximity labelling of the mammalian secretome. Open Biol. 2022 08; 12(8):220149. view on PubMed

Yang Z, Wang Y, Liu S, Deng W, Lomeli SH, Moriceau G, Wohlschlegel J, Piva M, Lo RS. Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discov. 2022 08 05; 12(8):1942-1959. view on PubMed

Mao Z, Nesterenko PA, McLaughlin J, Deng W, Burton Sojo G, Cheng D, Noguchi M, Chour W, DeLucia DC, Finton KA, Qin Y, Obusan MB, Tran W, Wang L, Bangayan NJ, Ta L, Chen CC, Seet CS, Crooks GM, Phillips JW, Heath JR, Strong RK, Lee JK, Wohlschlegel JA, Witte ON. Physical and in silico immunopeptidomic profiling of a cancer antigen prostatic acid phosphatase reveals targets enabling TCR isolation. Proc Natl Acad Sci U S A. 2022 08 02; 119(31):e2203410119. view on PubMed

Hernández-García J, Diego-Martin B, Kuo PH, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel J, Jacobsen SE, Blázquez MA, Gallego-Bartolomé J. Comprehensive identification of SWI/SNF complex subunits underpins deep eukaryotic ancestry and reveals new plant components. Commun Biol. 2022 06 06; 5(1):549. view on PubMed

Aguilera KY, Le T, Riahi R, Lay AR, Hinz S, Saadat EA, Vashisht AA, Wohlschlegel J, Donahue TR, Radu CG, Dawson DW. Porcupine Inhibition Disrupts Mitochondrial Function and Homeostasis in WNT Ligand-Addicted Pancreatic Cancer. Mol Cancer Ther. 2022 06 01; 21(6):936-947. view on PubMed

Fan X, Barshop WD, Vashisht AA, Pandey V, Leal S, Rayatpisheh S, Jami-Alahmadi Y, Sha J, Wohlschlegel JA. Iron-regulated assembly of the cytosolic iron-sulfur cluster biogenesis machinery. J Biol Chem. 2022 07; 298(7):102094. view on PubMed

Hebbar S, Panzade G, Vashisht AA, Wohlschlegel JA, Veksler-Lublinsky I, Zinovyeva AY. Functional identification of microRNA-centered complexes in C. elegans. Sci Rep. 2022 05 03; 12(1):7133. view on PubMed

Shen JZ, Qiu Z, Wu Q, Zhang G, Harris R, Sun D, Rantala J, Barshop WD, Zhao L, Lv D, Won KA, Wohlschlegel J, Sangfelt O, Laman H, Rich JN, Spruck C. A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell. 2022 03 17; 82(6):1123-1139.e8. view on PubMed

Potok ME, Zhong Z, Picard CL, Liu Q, Do T, Jacobsen CE, Sakr O, Naranbaatar B, Thilakaratne R, Khnkoyan Z, Purl M, Cheng H, Vervaet H, Feng S, Rayatpisheh S, Wohlschlegel JA, O'Malley RC, Ecker JR, Jacobsen SE. The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis. Proc Natl Acad Sci U S A. 2022 01 18; 119(3). view on PubMed

Muntean BS, Marwari S, Li X, Sloan DC, Young BD, Wohlschlegel JA, Martemyanov KA. Members of the KCTD family are major regulators of cAMP signaling. Proc Natl Acad Sci U S A. 2022 01 04; 119(1). view on PubMed

Salas N, Coceres VM, Melo TDS, Pereira-Neves A, Maguire VG, Rodriguez TM, Sabatke B, Ramirez MI, Sha J, Wohlschlegel JA, de Miguel N. VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles. Cell Mol Life Sci. 2021 Dec 24; 79(1):11. view on PubMed

Chern JH, Pasquarelli RR, Moon AS, Chen AL, Sha J, Wohlschlegel JA, Bradley PJ. A Novel Toxoplasma Inner Membrane Complex Suture-Associated Protein Regulates Suture Protein Targeting and Colocalizes with Membrane Trafficking Machinery. mBio. 2021 10 26; 12(5):e0245521. view on PubMed

Herbst WA, Deng W, Wohlschlegel JA, Achiro JM, Martin KC. Neuronal activity regulates the nuclear proteome to promote activity-dependent transcription. J Cell Biol. 2021 12 06; 220(12). view on PubMed

Falekun S, Sepulveda J, Jami-Alahmadi Y, Park H, Wohlschlegel JA, Sigala PA. Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites. Elife. 2021 10 06; 10. view on PubMed

Dietz JV, Willoughby MM, Piel RB, Ross TA, Bohovych I, Addis HG, Fox JL, Lanzilotta WN, Dailey HA, Wohlschlegel JA, Reddi AR, Medlock AE, Khalimonchuk O. Mitochondrial contact site and cristae organizing system (MICOS) machinery supports heme biosynthesis by enabling optimal performance of ferrochelatase. Redox Biol. 2021 10; 46:102125. view on PubMed

Wang H, Chan TW, Vashisht AA, Drew BG, Calkin AC, Harris TE, Wohlschlegel JA, Xiao X, Reue K. Lipin 1 modulates mRNA splicing during fasting adaptation in liver. JCI Insight. 2021 09 08; 6(17). view on PubMed

Zhang D, Dailey OR, Simon DJ, Roca-Datzer K, Jami-Alahmadi Y, Hennen MS, Wohlschlegel JA, Koehler CM, Dabir DV. Aim32 is a dual-localized 2Fe-2S mitochondrial protein that functions in redox quality control. J Biol Chem. 2021 10; 297(4):101135. view on PubMed

Young BD, Sha J, Vashisht AA, Wohlschlegel JA. Human Multisubunit E3 Ubiquitin Ligase Required for Heterotrimeric G-Protein β-Subunit Ubiquitination and Downstream Signaling. J Proteome Res. 2021 09 03; 20(9):4318-4330. view on PubMed

Clifford BL, Sedgeman LR, Williams KJ, Morand P, Cheng A, Jarrett KE, Chan AP, Brearley-Sholto MC, Wahlström A, Ashby JW, Barshop W, Wohlschlegel J, Calkin AC, Liu Y, Thorell A, Meikle PJ, Drew BG, Mack JJ, Marschall HU, Tarling EJ, Edwards PA, de Aguiar Vallim TQ. FXR activation protects against NAFLD via bile-acid-dependent reductions in lipid absorption. Cell Metab. 2021 08 03; 33(8):1671-1684.e4. view on PubMed

Xue Y, Zhong Z, Harris CJ, Gallego-Bartolomé J, Wang M, Picard C, Cao X, Hua S, Kwok I, Feng S, Jami-Alahmadi Y, Sha J, Gardiner J, Wohlschlegel J, Jacobsen SE. Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation. Nat Commun. 2021 07 13; 12(1):4292. view on PubMed

Martelly W, Fellows B, Kang P, Vashisht A, Wohlschlegel JA, Sharma S. Synergistic roles for human U1 snRNA stem-loops in pre-mRNA splicing. RNA Biol. 2021 12; 18(12):2576-2593. view on PubMed

Ichino L, Boone BA, Strauskulage L, Harris CJ, Kaur G, Gladstone MA, Tan M, Feng S, Jami-Alahmadi Y, Duttke SH, Wohlschlegel JA, Cheng X, Redding S, Jacobsen SE. MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO. Science. 2021 Jun 03. view on PubMed

Mayya VK, Flamand MN, Lambert AM, Jafarnejad SM, Wohlschlegel JA, Sonenberg N, Duchaine TF. microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development. Nucleic Acids Res. 2021 05 21; 49(9):4803-4815. view on PubMed

Jami-Alahmadi Y, Pandey V, Mayank AK, Wohlschlegel JA. A Robust Method for Packing High Resolution C18 RP-nano-HPLC Columns. J Vis Exp. 2021 05 14; (171). view on PubMed

Chen Y, Hu X, Liu S, Su T, Huang H, Ren H, Gao Z, Wang X, Lin D, Wohlschlegel JA, Wang Q, Lin C. Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ubiquitin ligases. Nat Commun. 2021 04 12; 12(1):2155. view on PubMed

Wendimu MY, Alqinyah M, Vella S, Dean P, Almutairi F, Davila-Rivera R, Rayatpisheh S, Wohlschlegel J, Moreno S, Hooks SB. RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate pro-inflammatory gene expression in microglia. Cell Signal. 2021 07; 83:109974. view on PubMed

Vélez-Ramírez DE, Shimogawa MM, Ray SS, Lopez A, Rayatpisheh S, Langousis G, Gallagher-Jones M, Dean S, Wohlschlegel JA, Hill KL. APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei. mSphere. 2021 02 10; 6(1). view on PubMed

Deng W, Sha J, Plath K, Wohlschlegel JA. Carboxylate-Modified Magnetic Bead (CMMB)-Based Isopropanol Gradient Peptide Fractionation (CIF) Enables Rapid and Robust Off-Line Peptide Mixture Fractionation in Bottom-Up Proteomics. Mol Cell Proteomics. 2021; 20:100039. view on PubMed

Nie M, Oravcová M, Jami-Alahmadi Y, Wohlschlegel JA, Lazzerini-Denchi E, Boddy MN. FAM111A induces nuclear dysfunction in disease and viral restriction. EMBO Rep. 2021 02 03; 22(2):e50803. view on PubMed

Shen JZ, Qiu Z, Wu Q, Finlay D, Garcia G, Sun D, Rantala J, Barshop W, Hope JL, Gimple RC, Sangfelt O, Bradley LM, Wohlschlegel J, Rich JN, Spruck C. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells. Cell. 2021 01 21; 184(2):352-369.e23. view on PubMed

Colozza G, Jami-Alahmadi Y, Dsouza A, Tejeda-Muñoz N, Albrecht LV, Sosa EA, Wohlschlegel JA, De Robertis EM. Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway. Sci Rep. 2020 12 09; 10(1):21555. view on PubMed

Poe AJ, Kulkarni M, Leszczynska A, Tang J, Shah R, Jami-Alahmadi Y, Wang J, Kramerov AA, Wohlschlegel J, Punj V, Ljubimov AV, Saghizadeh M. Integrated Transcriptome and Proteome Analyses Reveal the Regulatory Role of miR-146a in Human Limbal Epithelium via Notch Signaling. Cells. 2020 09 26; 9(10). view on PubMed

Geekiyanage H, Rayatpisheh S, Wohlschlegel JA, Brown R, Ambros V. Extracellular microRNAs in human circulation are associated with miRISC complexes that are accessible to anti-AGO2 antibody and can bind target mimic oligonucleotides. Proc Natl Acad Sci U S A. 2020 09 29; 117(39):24213-24223. view on PubMed

Sharma S, LeClaire M, Wohlschlegel J, Gimzewski J. Impact of isolation methods on the biophysical heterogeneity of single extracellular vesicles. Sci Rep. 2020 08 07; 10(1):13327. view on PubMed

Wang Y, Zhong Z, Zhang Y, Xu L, Feng S, Rayatpisheh S, Wohlschlegel JA, Wang Z, Jacobsen SE, Ausin I. NAP1-RELATED PROTEIN1 and 2 negatively regulate H2A.Z abundance in chromatin in Arabidopsis. Nat Commun. 2020 06 08; 11(1):2887. view on PubMed

Liu Q, Bischof S, Harris CJ, Zhong Z, Zhan L, Nguyen C, Rashoff A, Barshop WD, Sun F, Feng S, Potok M, Gallego-Bartolome J, Zhai J, Wohlschlegel JA, Carey MF, Long JA, Jacobsen SE. The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis. Nat Commun. 2020 06 03; 11(1):2798. view on PubMed

Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Hu X, Sha J, Wohlschlegel JA, Bradley PJ, Reese ML. Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis. Proc Natl Acad Sci U S A. 2020 06 02; 117(22):12164-12173. view on PubMed

Nadipuram SM, Thind AC, Rayatpisheh S, Wohlschlegel JA, Bradley PJ. Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites. PLoS One. 2020; 15(5):e0232552. view on PubMed

Lewis A, Berkyurek AC, Greiner A, Sawh AN, Vashisht A, Merrett S, Flamand MN, Wohlschlegel J, Sarov M, Miska EA, Duchaine TF. A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi. Mol Cell. 2020 06 04; 78(5):862-875.e8. view on PubMed

Nicolau M, Picault N, Descombin J, Jami-Alahmadi Y, Feng S, Bucher E, Jacobsen SE, Deragon JM, Wohlschlegel J, Moissiard G. The plant mobile domain proteins MAIN and MAIL1 interact with the phosphatase PP7L to regulate gene expression and silence transposable elements in Arabidopsis thaliana. PLoS Genet. 2020 04; 16(4):e1008324. view on PubMed

Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, Goldberg DE, Beck JR. EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole. Cell Microbiol. 2020 05; 22(5):e13168. view on PubMed

Grolla AA, Miggiano R, Di Marino D, Bianchi M, Gori A, Orsomando G, Gaudino F, Galli U, Del Grosso E, Mazzola F, Angeletti C, Guarneri M, Torretta S, Calabrò M, Boumya S, Fan X, Colombo G, Travelli C, Rocchio F, Aronica E, Wohlschlegel JA, Deaglio S, Rizzi M, Genazzani AA, Garavaglia S. A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus. J Biol Chem. 2020 03 13; 295(11):3635-3651. view on PubMed

Bradley PJ, Rayatpisheh S, Wohlschlegel JA, Nadipuram SM. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii. Methods Mol Biol. 2020; 2071:323-346. view on PubMed

Ho CM, Li X, Lai M, Terwilliger TC, Beck JR, Wohlschlegel J, Goldberg DE, Fitzpatrick AWP, Zhou ZH. Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu. Nat Methods. 2020 01; 17(1):79-85. view on PubMed

Choi CP, Moon AS, Back PS, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel JA, Bradley PJ. A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex. PLoS Biol. 2019 10; 17(10):e3000475. view on PubMed

Barshop WD, Kim HJ, Fan X, Sha J, Rayatpisheh S, Wohlschlegel JA. Chemical Derivatization of Affinity Matrices Provides Protection from Tryptic Proteolysis. J Proteome Res. 2019 10 04; 18(10):3586-3596. view on PubMed

Hu X, Weston TA, He C, Jung RS, Heizer PJ, Young BD, Tu Y, Tontonoz P, Wohlschlegel JA, Jiang H, Young SG, Fong LG. Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia. Elife. 2019 09 05; 8. view on PubMed

Wongpalee SP, Liu S, Gallego-Bartolomé J, Leitner A, Aebersold R, Liu W, Yen L, Nohales MA, Kuo PH, Vashisht AA, Wohlschlegel JA, Feng S, Kay SA, Zhou ZH, Jacobsen SE. CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nat Commun. 2019 09 02; 10(1):3916. view on PubMed

Patel SJ, Frey AG, Palenchar DJ, Achar S, Bullough KZ, Vashisht A, Wohlschlegel JA, Philpott CC. A PCBP1-BolA2 chaperone complex delivers iron for cytosolic [2Fe-2S] cluster assembly. Nat Chem Biol. 2019 09; 15(9):872-881. view on PubMed

Potok ME, Wang Y, Xu L, Zhong Z, Liu W, Feng S, Naranbaatar B, Rayatpisheh S, Wang Z, Wohlschlegel JA, Ausin I, Jacobsen SE. Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition. Nat Commun. 2019 07 26; 10(1):3352. view on PubMed

Mayank AK, Pandey V, Vashisht AA, Barshop WD, Rayatpisheh S, Sharma T, Haque T, Powers DN, Wohlschlegel JA. An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis. Mol Cell. 2019 07 25; 75(2):382-393.e5. view on PubMed

Mota CM, Oliveira ACM, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TWP. Corrigendum: Neospora Caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity. Front Microbiol. 2019; 10:548. view on PubMed

Barshop WD, Rayatpisheh S, Kim HJ, Wohlschlegel JA. Sequential Windowed Acquisition of Reporter Masses for Quantitation-First Proteomics. J Proteome Res. 2019 04 05; 18(4):1893-1901. view on PubMed

Heinzelman P, Powers DN, Wohlschlegel JA, John V. Shotgun Proteomic Profiling of Bloodborne Nanoscale Extracellular Vesicles. Methods Mol Biol. 2019; 1897:403-416. view on PubMed

Quintero D, Carrafa J, Vincent L, Lee HJ, Wohlschlegel J, Bermudes D. Co-Expression of a Chimeric Protease Inhibitor Secreted by a Tumor-Targeted Salmonella Protects Therapeutic Proteins from Proteolytic Degradation. J Microbiol Biotechnol. 2018 Dec 28; 28(12):2079-2094. view on PubMed

Lao JP, Ulrich KM, Johnson JR, Newton BW, Vashisht AA, Wohlschlegel JA, Krogan NJ, Toczyski DP. The Yeast DNA Damage Checkpoint Kinase Rad53 Targets the Exoribonuclease, Xrn1. G3 (Bethesda). 2018 12 10; 8(12):3931-3944. view on PubMed

Harris CJ, Scheibe M, Wongpalee SP, Liu W, Cornett EM, Vaughan RM, Li X, Chen W, Xue Y, Zhong Z, Yen L, Barshop WD, Rayatpisheh S, Gallego-Bartolome J, Groth M, Wang Z, Wohlschlegel JA, Du J, Rothbart SB, Butter F, Jacobsen SE. A DNA methylation reader complex that enhances gene transcription. Science. 2018 12 07; 362(6419):1182-1186. view on PubMed

Metz KA, Teng X, Coppens I, Lamb HM, Wagner BE, Rosenfeld JA, Chen X, Zhang Y, Kim HJ, Meadow ME, Wang TS, Haberlandt ED, Anderson GW, Leshinsky-Silver E, Bi W, Markello TC, Pratt M, Makhseed N, Garnica A, Danylchuk NR, Burrow TA, Jayakar P, McKnight D, Agadi S, Gbedawo H, Stanley C, Alber M, Prehl I, Peariso K, Ong MT, Mordekar SR, Parker MJ, Crooks D, Agrawal PB, Berry GT, Loddenkemper T, Yang Y, Maegawa GHB, Aouacheria A, Markle JG, Wohlschlegel JA, Hartman AL, Hardwick JM. KCTD7 deficiency defines a distinct neurodegenerative disorder with a conserved autophagy-lysosome defect. Ann Neurol. 2018 11; 84(5):766-780. view on PubMed

Romero AM, Martínez-Pastor M, Du G, Solé C, Carlos M, Vergara SV, Sanvisens N, Wohlschlegel JA, Toczyski DP, Posas F, de Nadal E, Martínez-Pastor MT, Thiele DJ, Puig S. Phosphorylation and Proteasome Recognition of the mRNA-Binding Protein Cth2 Facilitates Yeast Adaptation to Iron Deficiency. mBio. 2018 09 18; 9(5). view on PubMed

Kerins MJ, Milligan J, Wohlschlegel JA, Ooi A. Fumarate hydratase inactivation in hereditary leiomyomatosis and renal cell cancer is synthetic lethal with ferroptosis induction. Cancer Sci. 2018 Sep; 109(9):2757-2766. view on PubMed

Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. Retraction Note: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins. Sci Rep. 2018 07 13; 8(1):10964. view on PubMed

Lee JK, Bangayan NJ, Chai T, Smith BA, Pariva TE, Yun S, Vashisht A, Zhang Q, Park JW, Corey E, Huang J, Graeber TG, Wohlschlegel J, Witte ON. Systemic surfaceome profiling identifies target antigens for immune-based therapy in subtypes of advanced prostate cancer. Proc Natl Acad Sci U S A. 2018 05 08; 115(19):E4473-E4482. view on PubMed

Benador IY, Veliova M, Mahdaviani K, Petcherski A, Wikstrom JD, Assali EA, Acín-Pérez R, Shum M, Oliveira MF, Cinti S, Sztalryd C, Barshop WD, Wohlschlegel JA, Corkey BE, Liesa M, Shirihai OS. Mitochondria Bound to Lipid Droplets Have Unique Bioenergetics, Composition, and Dynamics that Support Lipid Droplet Expansion. Cell Metab. 2018 04 03; 27(4):869-885.e6. view on PubMed

Nievas YR, Vashisht AA, Corvi MM, Metz S, Johnson PJ, Wohlschlegel JA, de Miguel N. Protein Palmitoylation Plays an Important Role in Trichomonas vaginalis Adherence. Mol Cell Proteomics. 2018 11; 17(11):2229-2241. view on PubMed

Stehling O, Mascarenhas J, Vashisht AA, Sheftel AD, Niggemeyer B, Rösser R, Pierik AJ, Wohlschlegel JA, Lill R. Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins. Cell Metab. 2018 01 09; 27(1):263. view on PubMed

Freire ER, Moura DMN, Bezerra MJR, Xavier CC, Morais-Sobral MC, Vashisht AA, Rezende AM, Wohlschlegel JA, Sturm NR, de Melo Neto OP, Campbell DA. Trypanosoma brucei EIF4E2 cap-binding protein binds a homolog of the histone-mRNA stem-loop-binding protein. Curr Genet. 2018 Aug; 64(4):821-839. view on PubMed

Nievas YR, Coceres VM, Midlej V, de Souza W, Benchimol M, Pereira-Neves A, Vashisht AA, Wohlschlegel JA, Johnson PJ, de Miguel N. Membrane-shed vesicles from the parasite Trichomonas vaginalis: characterization and their association with cell interaction. Cell Mol Life Sci. 2018 06; 75(12):2211-2226. view on PubMed

Rengasamy M, Zhang F, Vashisht A, Song WM, Aguilo F, Sun Y, Li S, Zhang W, Zhang B, Wohlschlegel JA, Walsh MJ. The PRMT5/WDR77 complex regulates alternative splicing through ZNF326 in breast cancer. Nucleic Acids Res. 2017 Nov 02; 45(19):11106-11120. view on PubMed

Zhang Z, Ren P, Vashisht AA, Wohlschlegel JA, Quintana DG, Zeng F. Cdk1-interacting protein Cip1 is regulated by the S phase checkpoint in response to genotoxic stress. Genes Cells. 2017 Oct; 22(10):850-860. view on PubMed

Xue Y, Pradhan SK, Sun F, Chronis C, Tran N, Su T, Van C, Vashisht A, Wohlschlegel J, Peterson CL, Timmers HTM, Kurdistani SK, Carey MF. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell. 2017 Aug 17; 67(4):594-607.e4. view on PubMed

Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. New molecular tools in Neospora caninum for studying apicomplexan parasite proteins. Sci Rep. 2017 06 19; 7(1):3768. view on PubMed

Fetherolf MM, Boyd SD, Taylor AB, Kim HJ, Wohlschlegel JA, Blackburn NJ, Hart PJ, Winge DR, Winkler DD. Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site. J Biol Chem. 2017 07 21; 292(29):12025-12040. view on PubMed

Kerins MJ, Vashisht AA, Liang BX, Duckworth SJ, Praslicka BJ, Wohlschlegel JA, Ooi A. Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes. Mol Cell Biol. 2017 06 01; 37(11). view on PubMed

Liu Q, Wang Q, Deng W, Wang X, Piao M, Cai D, Li Y, Barshop WD, Yu X, Zhou T, Liu B, Oka Y, Wohlschlegel J, Zuo Z, Lin C. Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2. Nat Commun. 2017 05 11; 8:15234. view on PubMed

Li D, Palanca AMS, Won SY, Gao L, Feng Y, Vashisht AA, Liu L, Zhao Y, Liu X, Wu X, Li S, Le B, Kim YJ, Yang G, Li S, Liu J, Wohlschlegel JA, Guo H, Mo B, Chen X, Law JA. The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status. Elife. 2017 04 28; 6. view on PubMed

Wu E, Vashisht AA, Chapat C, Flamand MN, Cohen E, Sarov M, Tabach Y, Sonenberg N, Wohlschlegel J, Duchaine TF. A continuum of mRNP complexes in embryonic microRNA-mediated silencing. Nucleic Acids Res. 2017 02 28; 45(4):2081-2098. view on PubMed

Wang Q, Barshop WD, Bian M, Vashisht AA, He R, Yu X, Liu B, Nguyen P, Liu X, Zhao X, Wohlschlegel JA, Lin C. The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2. Mol Plant. 2017 02 13; 10(2):357. view on PubMed

Huang C, Su T, Xue Y, Cheng C, Lay FD, McKee RA, Li M, Vashisht A, Wohlschlegel J, Novitch BG, Plath K, Kurdistani SK, Carey M. Cbx3 maintains lineage specificity during neural differentiation. Genes Dev. 2017 02 01; 31(3):241-246. view on PubMed

Steffen J, Vashisht AA, Wan J, Jen JC, Claypool SM, Wohlschlegel JA, Koehler CM. Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. Mol Biol Cell. 2017 Mar 01; 28(5):600-612. view on PubMed

Wongpalee SP, Vashisht A, Sharma S, Chui D, Wohlschlegel JA, Black DL. Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing. Elife. 2016 11 24; 5. view on PubMed

Chen AL, Moon AS, Bell HN, Huang AS, Vashisht AA, Toh JY, Lin AH, Nadipuram SM, Kim EW, Choi CP, Wohlschlegel JA, Bradley PJ. Novel insights into the composition and function of the Toxoplasma IMC sutures. Cell Microbiol. 2017 04; 19(4). view on PubMed

Nitarska J, Smith JG, Sherlock WT, Hillege MM, Nott A, Barshop WD, Vashisht AA, Wohlschlegel JA, Mitter R, Riccio A. A Functional Switch of NuRD Chromatin Remodeling Complex Subunits Regulates Mouse Cortical Development. Cell Rep. 2016 11 01; 17(6):1683-1698. view on PubMed

Wang Q, Zuo Z, Wang X, Gu L, Yoshizumi T, Yang Z, Yang L, Liu Q, Liu W, Han YJ, Kim JI, Liu B, Wohlschlegel JA, Matsui M, Oka Y, Lin C. Photoactivation and inactivation of Arabidopsis cryptochrome 2. Science. 2016 10 21; 354(6310):343-347. view on PubMed

Caslavka Zempel KE, Vashisht AA, Barshop WD, Wohlschlegel JA, Clarke SG. Determining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC. J Proteome Res. 2016 12 02; 15(12):4436-4451. view on PubMed

Mota CM, Oliveira AC, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TW. Neospora caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity. Front Microbiol. 2016; 7:1456. view on PubMed

Piel RB, Shiferaw MT, Vashisht AA, Marcero JR, Praissman JL, Phillips JD, Wohlschlegel JA, Medlock AE. A Novel Role for Progesterone Receptor Membrane Component 1 (PGRMC1): A Partner and Regulator of Ferrochelatase. Biochemistry. 2016 09 20; 55(37):5204-17. view on PubMed

Melber A, Na U, Vashisht A, Weiler BD, Lill R, Wohlschlegel JA, Winge DR. Role of Nfu1 and Bol3 in iron-sulfur cluster transfer to mitochondrial clients. Elife. 2016 08 17; 5. view on PubMed

Drake JM, Paull EO, Graham NA, Lee JK, Smith BA, Titz B, Stoyanova T, Faltermeier CM, Uzunangelov V, Carlin DE, Fleming DT, Wong CK, Newton Y, Sudha S, Vashisht AA, Huang J, Wohlschlegel JA, Graeber TG, Witte ON, Stuart JM. Phosphoproteome Integration Reveals Patient-Specific Networks in Prostate Cancer. Cell. 2016 Aug 11; 166(4):1041-1054. view on PubMed

Nadipuram SM, Kim EW, Vashisht AA, Lin AH, Bell HN, Coppens I, Wohlschlegel JA, Bradley PJ. In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis. mBio. 2016 08 02; 7(4). view on PubMed

Li F, Bullough KZ, Vashisht AA, Wohlschlegel JA, Philpott CC. Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells. Mol Cell Biol. 2016 08 15; 36(16):2121-31. view on PubMed

Harris CJ, Husmann D, Liu W, Kasmi FE, Wang H, Papikian A, Pastor WA, Moissiard G, Vashisht AA, Dangl JL, Wohlschlegel JA, Jacobsen SE. Arabidopsis AtMORC4 and AtMORC7 Form Nuclear Bodies and Repress a Large Number of Protein-Coding Genes. PLoS Genet. 2016 05; 12(5):e1005998. view on PubMed

Damianov A, Ying Y, Lin CH, Lee JA, Tran D, Vashisht AA, Bahrami-Samani E, Xing Y, Martin KC, Wohlschlegel JA, Black DL. Rbfox Proteins Regulate Splicing as Part of a Large Multiprotein Complex LASR. Cell. 2016 Apr 21; 165(3):606-19. view on PubMed

Flamand MN, Wu E, Vashisht A, Jannot G, Keiper BD, Simard MJ, Wohlschlegel J, Duchaine TF. Poly(A)-binding proteins are required for microRNA-mediated silencing and to promote target deadenylation in C. elegans. Nucleic Acids Res. 2016 07 08; 44(12):5924-35. view on PubMed

Kwong PN, Chambers M, Vashisht AA, Turki-Judeh W, Yau TY, Wohlschlegel JA, Courey AJ. The central region of the Drosophila co-repressor Groucho as a regulatory hub. J Biol Chem. 2016 Mar 25; 291(13):6784. view on PubMed

Kim EW, Nadipuram SM, Tetlow AL, Barshop WD, Liu PT, Wohlschlegel JA, Bradley PJ. The Rhoptry Pseudokinase ROP54 Modulates Toxoplasma gondii Virulence and Host GBP2 Loading. mSphere. 2016 Mar-Apr; 1(2). view on PubMed

Kumar V, Madina BR, Gulati S, Vashisht AA, Kanyumbu C, Pieters B, Shakir A, Wohlschlegel JA, Read LK, Mooers BHM, Cruz-Reyes J. REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. J Biol Chem. 2016 Mar 11; 291(11):5753-5764. view on PubMed

Aguilo F, Li S, Balasubramaniyan N, Sancho A, Benko S, Zhang F, Vashisht A, Rengasamy M, Andino B, Chen CH, Zhou F, Qian C, Zhou MM, Wohlschlegel JA, Zhang W, Suchy FJ, Walsh MJ. Deposition of 5-Methylcytosine on Enhancer RNAs Enables the Coactivator Function of PGC-1α. Cell Rep. 2016 Jan 26; 14(3):479-492. view on PubMed

Langousis G, Shimogawa MM, Saada EA, Vashisht AA, Spreafico R, Nager AR, Barshop WD, Nachury MV, Wohlschlegel JA, Hill KL. Loss of the BBSome perturbs endocytic trafficking and disrupts virulence of Trypanosoma brucei. Proc Natl Acad Sci U S A. 2016 Jan 19; 113(3):632-7. view on PubMed

Berg JM, Lee C, Chen L, Galvan L, Cepeda C, Chen JY, Peñagarikano O, Stein JL, Li A, Oguro-Ando A, Miller JA, Vashisht AA, Starks ME, Kite EP, Tam E, Gdalyahu A, Al-Sharif NB, Burkett ZD, White SA, Fears SC, Levine MS, Wohlschlegel JA, Geschwind DH. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. Neuron. 2015 Dec 16; 88(6):1173-1191. view on PubMed

Aguilo F, Zhang F, Sancho A, Fidalgo M, Di Cecilia S, Vashisht A, Lee DF, Chen CH, Rengasamy M, Andino B, Jahouh F, Roman A, Krig SR, Wang R, Zhang W, Wohlschlegel JA, Wang J, Walsh MJ. Coordination of m(6)A mRNA Methylation and Gene Transcription by ZFP217 Regulates Pluripotency and Reprogramming. Cell Stem Cell. 2015 Dec 03; 17(6):689-704. view on PubMed

Kwong PN, Chambers M, Vashisht AA, Turki-Judeh W, Yau TY, Wohlschlegel JA, Courey AJ. The Central Region of the Drosophila Co-repressor Groucho as a Regulatory Hub. J Biol Chem. 2015 Dec 11; 290(50):30119-30. view on PubMed

Nie M, Vashisht AA, Wohlschlegel JA, Boddy MN. High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification. Sci Rep. 2015 Sep 25; 5:14389. view on PubMed

Zinovyeva AY, Veksler-Lublinsky I, Vashisht AA, Wohlschlegel JA, Ambros VR. Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal. Proc Natl Acad Sci U S A. 2015 Sep 22; 112(38):E5271-80. view on PubMed

Ch'ng TH, DeSalvo M, Lin P, Vashisht A, Wohlschlegel JA, Martin KC. Cell biological mechanisms of activity-dependent synapse to nucleus translocation of CRTC1 in neurons. Front Mol Neurosci. 2015; 8:48. view on PubMed

Palou G, Palou R, Zeng F, Vashisht AA, Wohlschlegel JA, Quintana DG. Three Different Pathways Prevent Chromosome Segregation in the Presence of DNA Damage or Replication Stress in Budding Yeast. PLoS Genet. 2015 Sep; 11(9):e1005468. view on PubMed

Medlock AE, Shiferaw MT, Marcero JR, Vashisht AA, Wohlschlegel JA, Phillips JD, Dailey HA. Identification of the Mitochondrial Heme Metabolism Complex. PLoS One. 2015; 10(8):e0135896. view on PubMed

Goodwin JF, Kothari V, Drake JM, Zhao S, Dylgjeri E, Dean JL, Schiewer MJ, McNair C, Jones JK, Aytes A, Magee MS, Snook AE, Zhu Z, Den RB, Birbe RC, Gomella LG, Graham NA, Vashisht AA, Wohlschlegel JA, Graeber TG, Karnes RJ, Takhar M, Davicioni E, Tomlins SA, Abate-Shen C, Sharifi N, Witte ON, Feng FY, Knudsen KE. DNA-PKcs-Mediated Transcriptional Regulation Drives Prostate Cancer Progression and Metastasis. Cancer Cell. 2015 Jul 13; 28(1):97-113. view on PubMed

Loveless TB, Topacio BR, Vashisht AA, Galaang S, Ulrich KM, Young BD, Wohlschlegel JA, Toczyski DP. DNA Damage Regulates Translation through β-TRCP Targeting of CReP. PLoS Genet. 2015 Jun; 11(6):e1005292. view on PubMed

Shimogawa MM, Saada EA, Vashisht AA, Barshop WD, Wohlschlegel JA, Hill KL. Cell Surface Proteomics Provides Insight into Stage-Specific Remodeling of the Host-Parasite Interface in Trypanosoma brucei. Mol Cell Proteomics. 2015 Jul; 14(7):1977-88. view on PubMed

Zhang S, Zhou B, Kang Y, Cui X, Liu A, Deleris A, Greenberg MV, Cui X, Qiu Q, Lu F, Wohlschlegel JA, Jacobsen SE, Cao X. C-terminal domains of a histone demethylase interact with a pair of transcription factors and mediate specific chromatin association. Cell Discov. 2015; 1. view on PubMed

Vashisht AA, Yu CC, Sharma T, Ro K, Wohlschlegel JA. The Association of the Xeroderma Pigmentosum Group D DNA Helicase (XPD) with Transcription Factor IIH Is Regulated by the Cytosolic Iron-Sulfur Cluster Assembly Pathway. J Biol Chem. 2015 May 29; 290(22):14218-25. view on PubMed

Pentecost M, Vashisht AA, Lester T, Voros T, Beaty SM, Park A, Wang YE, Yun TE, Freiberg AN, Wohlschlegel JA, Lee B. Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins. PLoS Pathog. 2015 Mar; 11(3):e1004739. view on PubMed

Wang Q, Barshop WD, Bian M, Vashisht AA, He R, Yu X, Liu B, Nguyen P, Liu X, Zhao X, Wohlschlegel JA, Lin C. The blue light-dependent phosphorylation of the CCE domain determines the photosensitivity of Arabidopsis CRY2. Mol Plant. 2015 04; 8(4):631-43. view on PubMed

Chen AL, Kim EW, Toh JY, Vashisht AA, Rashoff AQ, Van C, Huang AS, Moon AS, Bell HN, Bentolila LA, Wohlschlegel JA, Bradley PJ. Novel components of the Toxoplasma inner membrane complex revealed by BioID. mBio. 2015 Feb 17; 6(1):e02357-14. view on PubMed

Xue Y, Van C, Pradhan SK, Su T, Gehrke J, Kuryan BG, Kitada T, Vashisht A, Tran N, Wohlschlegel J, Peterson CL, Kurdistani SK, Carey MF. The Ino80 complex prevents invasion of euchromatin into silent chromatin. Genes Dev. 2015 Feb 15; 29(4):350-5. view on PubMed

Sancho A, Li S, Paul T, Zhang F, Aguilo F, Vashisht A, Balasubramaniyan N, Leleiko NS, Suchy FJ, Wohlschlegel JA, Zhang W, Walsh MJ. CHD6 regulates the topological arrangement of the CFTR locus. Hum Mol Genet. 2015 May 15; 24(10):2724-32. view on PubMed

Jonas SJ, Stieg AZ, Richardson W, Guo S, Powers DN, Wohlschlegel J, Dunn B. Protein Adsorption Alters Hydrophobic Surfaces Used for Suspension Culture of Pluripotent Stem Cells. J Phys Chem Lett. 2015 Feb 05; 6(3):388-93. view on PubMed

Zhong X, Hale CJ, Nguyen M, Ausin I, Groth M, Hetzel J, Vashisht AA, Henderson IR, Wohlschlegel JA, Jacobsen SE. Domains rearranged methyltransferase3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc Natl Acad Sci U S A. 2015 Jan 20; 112(3):911-6. view on PubMed

Senese S, Cheung K, Lo YC, Gholkar AA, Xia X, Wohlschlegel JA, Torres JZ. A unique insertion in STARD9's motor domain regulates its stability. Mol Biol Cell. 2015 Feb 01; 26(3):440-52. view on PubMed

Groth M, Stroud H, Feng S, Greenberg MV, Vashisht AA, Wohlschlegel JA, Jacobsen SE, Ausin I. SNF2 chromatin remodeler-family proteins FRG1 and -2 are required for RNA-directed DNA methylation. Proc Natl Acad Sci U S A. 2014 Dec 09; 111(49):17666-71. view on PubMed

Sharma S, Wongpalee SP, Vashisht A, Wohlschlegel JA, Black DL. Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly. Genes Dev. 2014 Nov 15; 28(22):2518-31. view on PubMed

Du J, Johnson LM, Groth M, Feng S, Hale CJ, Li S, Vashisht AA, Wohlschlegel JA, Patel DJ, Jacobsen SE. Mechanism of DNA methylation-directed histone methylation by KRYPTONITE. Mol Cell. 2014 Aug 07; 55(3):495-504. view on PubMed

Zilio N, Codlin S, Vashisht AA, Bitton DA, Head SR, Wohlschlegel JA, Bähler J, Boddy MN. A novel histone deacetylase complex in the control of transcription and genome stability. Mol Cell Biol. 2014 Sep 15; 34(18):3500-14. view on PubMed

Freire ER, Vashisht AA, Malvezzi AM, Zuberek J, Langousis G, Saada EA, Nascimento Jde F, Stepinski J, Darzynkiewicz E, Hill K, De Melo Neto OP, Wohlschlegel JA, Sturm NR, Campbell DA. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei. RNA. 2014 Aug; 20(8):1272-86. view on PubMed

Saada EA, Kabututu ZP, Lopez M, Shimogawa MM, Langousis G, Oberholzer M, Riestra A, Jonsson ZO, Wohlschlegel JA, Hill KL. Insect stage-specific receptor adenylate cyclases are localized to distinct subdomains of the Trypanosoma brucei Flagellar membrane. Eukaryot Cell. 2014 Aug; 13(8):1064-76. view on PubMed

Zhong X, Du J, Hale CJ, Gallego-Bartolome J, Feng S, Vashisht AA, Chory J, Wohlschlegel JA, Patel DJ, Jacobsen SE. Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell. 2014 May 22; 157(5):1050-60. view on PubMed

Freire ER, Malvezzi AM, Vashisht AA, Zuberek J, Saada EA, Langousis G, Nascimento JD, Moura D, Darzynkiewicz E, Hill K, de Melo Neto OP, Wohlschlegel JA, Sturm NR, Campbell DA. Trypanosoma brucei translation initiation factor homolog EIF4E6 forms a tripartite cytosolic complex with EIF4G5 and a capping enzyme homolog. Eukaryot Cell. 2014 Jul; 13(7):896-908. view on PubMed

Moissiard G, Bischof S, Husmann D, Pastor WA, Hale CJ, Yen L, Stroud H, Papikian A, Vashisht AA, Wohlschlegel JA, Jacobsen SE. Transcriptional gene silencing by Arabidopsis microrchidia homologues involves the formation of heteromers. Proc Natl Acad Sci U S A. 2014 May 20; 111(20):7474-9. view on PubMed

Edenberg ER, Vashisht AA, Topacio BR, Wohlschlegel JA, Toczyski DP. Hst3 is turned over by a replication stress-responsive SCF(Cdc4) phospho-degron. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5962-7. view on PubMed

Xue Y, Vashisht AA, Tan Y, Su T, Wohlschlegel JA. PRB1 is required for clipping of the histone H3 N terminal tail in Saccharomyces cerevisiae. PLoS One. 2014; 9(2):e90496. view on PubMed

Tan Y, Sun D, Jiang W, Klotz-Noack K, Vashisht AA, Wohlschlegel J, Widschwendter M, Spruck C. PP2A-B55β antagonizes cyclin E1 proteolysis and promotes its dysregulation in cancer. Cancer Res. 2014 Apr 01; 74(7):2006-14. view on PubMed

Edenberg ER, Vashisht A, Benanti JA, Wohlschlegel J, Toczyski DP. Rad53 downregulates mitotic gene transcription by inhibiting the transcriptional activator Ndd1. Mol Cell Biol. 2014 Feb; 34(4):725-38. view on PubMed

Vandeput F, Szabo-Fresnais N, Ahmad F, Kho C, Lee A, Krall J, Dunlop A, Hazel MW, Wohlschlegel JA, Hajjar RJ, Houslay MD, Manganiello VC, Movsesian MA. Selective regulation of cyclic nucleotide phosphodiesterase PDE3A isoforms. Proc Natl Acad Sci U S A. 2013 Dec 03; 110(49):19778-83. view on PubMed

Downey M, Knight B, Vashisht AA, Seller CA, Wohlschlegel JA, Shore D, Toczyski DP. Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1. Curr Biol. 2013 Sep 09; 23(17):1638-48. view on PubMed

Stehling O, Mascarenhas J, Vashisht AA, Sheftel AD, Niggemeyer B, Rösser R, Pierik AJ, Wohlschlegel JA, Lill R. Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins. Cell Metab. 2013 Aug 06; 18(2):187-98. view on PubMed

Twu O, de Miguel N, Lustig G, Stevens GC, Vashisht AA, Wohlschlegel JA, Johnson PJ. Trichomonas vaginalis exosomes deliver cargo to host cells and mediate host∶parasite interactions. PLoS Pathog. 2013; 9(7):e1003482. view on PubMed

Pan YR, Sun M, Wohlschlegel J, Reed SI. Cks1 enhances transcription efficiency at the GAL1 locus by linking the Paf1 complex to the 19S proteasome. Eukaryot Cell. 2013 Sep; 12(9):1192-201. view on PubMed

Cepeda D, Ng HF, Sharifi HR, Mahmoudi S, Cerrato VS, Fredlund E, Magnusson K, Nilsson H, Malyukova A, Rantala J, Klevebring D, Viñals F, Bhaskaran N, Zakaria SM, Rahmanto AS, Grotegut S, Nielsen ML, Szigyarto CA, Sun D, Lerner M, Navani S, Widschwendter M, Uhlén M, Jirström K, Pontén F, Wohlschlegel J, Grandér D, Spruck C, Larsson LG, Sangfelt O. CDK-mediated activation of the SCF(FBXO) (28) ubiquitin ligase promotes MYC-driven transcription and tumourigenesis and predicts poor survival in breast cancer. EMBO Mol Med. 2013 Jul; 5(7):1067-86. view on PubMed

Lee YJ, Lee CY, Grzechnik A, Gonzales-Zubiate F, Vashisht AA, Lee A, Wohlschlegel J, Chanfreau GF. RNA polymerase I stability couples cellular growth to metal availability. Mol Cell. 2013 Jul 11; 51(1):105-15. view on PubMed

Dehé PM, Coulon S, Scaglione S, Shanahan P, Takedachi A, Wohlschlegel JA, Yates JR, Llorente B, Russell P, Gaillard PH. Regulation of Mus81-Eme1 Holliday junction resolvase in response to DNA damage. Nat Struct Mol Biol. 2013 May; 20(5):598-603. view on PubMed

Beck JR, Fung C, Straub KW, Coppens I, Vashisht AA, Wohlschlegel JA, Bradley PJ. A Toxoplasma palmitoyl acyl transferase and the palmitoylated armadillo repeat protein TgARO govern apical rhoptry tethering and reveal a critical role for the rhoptries in host cell invasion but not egress. PLoS Pathog. 2013 Feb; 9(2):e1003162. view on PubMed

Kim MS, Machida Y, Vashisht AA, Wohlschlegel JA, Pang YP, Machida YJ. Regulation of error-prone translesion synthesis by Spartan/C1orf124. Nucleic Acids Res. 2013 Feb 01; 41(3):1661-8. view on PubMed

Chen XF, Lehmann L, Lin JJ, Vashisht A, Schmidt R, Ferrari R, Huang C, McKee R, Mosley A, Plath K, Kurdistani SK, Wohlschlegel J, Carey M. Mediator and SAGA have distinct roles in Pol II preinitiation complex assembly and function. Cell Rep. 2012 Nov 29; 2(5):1061-7. view on PubMed

Caro E, Stroud H, Greenberg MV, Bernatavichute YV, Feng S, Groth M, Vashisht AA, Wohlschlegel J, Jacobsen SE. The SET-domain protein SUVR5 mediates H3K9me2 deposition and silencing at stimulus response genes in a DNA methylation-independent manner. PLoS Genet. 2012; 8(10):e1002995. view on PubMed

Sun Y, Vashisht AA, Tchieu J, Wohlschlegel JA, Dreier L. Voltage-dependent anion channels (VDACs) recruit Parkin to defective mitochondria to promote mitochondrial autophagy. J Biol Chem. 2012 Nov 23; 287(48):40652-60. view on PubMed

Du J, Zhong X, Bernatavichute YV, Stroud H, Feng S, Caro E, Vashisht AA, Terragni J, Chin HG, Tu A, Hetzel J, Wohlschlegel JA, Pradhan S, Patel DJ, Jacobsen SE. Dual binding of chromomethylase domains to H3K9me2-containing nucleosomes directs DNA methylation in plants. Cell. 2012 Sep 28; 151(1):167-80. view on PubMed

Lehmann L, Ferrari R, Vashisht AA, Wohlschlegel JA, Kurdistani SK, Carey M. Polycomb repressive complex 1 (PRC1) disassembles RNA polymerase II preinitiation complexes. J Biol Chem. 2012 Oct 19; 287(43):35784-94. view on PubMed

Nie M, Aslanian A, Prudden J, Heideker J, Vashisht AA, Wohlschlegel JA, Yates JR, Boddy MN. Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions. J Biol Chem. 2012 Aug 24; 287(35):29610-9. view on PubMed

Stehling O, Vashisht AA, Mascarenhas J, Jonsson ZO, Sharma T, Netz DJ, Pierik AJ, Wohlschlegel JA, Lill R. MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science. 2012 Jul 13; 337(6091):195-9. view on PubMed

Ausin I, Greenberg MV, Simanshu DK, Hale CJ, Vashisht AA, Simon SA, Lee TF, Feng S, Española SD, Meyers BC, Wohlschlegel JA, Patel DJ, Jacobsen SE. INVOLVED IN DE NOVO 2-containing complex involved in RNA-directed DNA methylation in Arabidopsis. Proc Natl Acad Sci U S A. 2012 May 29; 109(22):8374-81. view on PubMed

Thivierge C, Makil N, Flamand M, Vasale JJ, Mello CC, Wohlschlegel J, Conte D, Duchaine TF. Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi. Nat Struct Mol Biol. 2011 Dec 18; 19(1):90-7. view on PubMed

Lomenick B, Jung G, Wohlschlegel JA, Huang J. Target identification using drug affinity responsive target stability (DARTS). Curr Protoc Chem Biol. 2011 Dec 01; 3(4):163-180. view on PubMed

Lin JJ, Lehmann LW, Bonora G, Sridharan R, Vashisht AA, Tran N, Plath K, Wohlschlegel JA, Carey M. Mediator coordinates PIC assembly with recruitment of CHD1. Genes Dev. 2011 Oct 15; 25(20):2198-209. view on PubMed

Madina BR, Kuppan G, Vashisht AA, Liang YH, Downey KM, Wohlschlegel JA, Ji X, Sze SH, Sacchettini JC, Read LK, Cruz-Reyes J. Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei. RNA. 2011 Oct; 17(10):1821-30. view on PubMed

Law JA, Vashisht AA, Wohlschlegel JA, Jacobsen SE. SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. PLoS Genet. 2011 Jul; 7(7):e1002195. view on PubMed

Oberholzer M, Langousis G, Nguyen HT, Saada EA, Shimogawa MM, Jonsson ZO, Nguyen SM, Wohlschlegel JA, Hill KL. Independent analysis of the flagellum surface and matrix proteomes provides insight into flagellum signaling in mammalian-infectious Trypanosoma brucei. Mol Cell Proteomics. 2011 Oct; 10(10):M111.010538. view on PubMed

Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A. Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. Mol Cell Biol. 2011 Aug; 31(15):3136-45. view on PubMed

Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN. DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes. Mol Cell Biol. 2011 Jun; 31(11):2299-310. view on PubMed

Smith AJ, Chudnovsky L, Simoes-Barbosa A, Delgadillo-Correa MG, Jonsson ZO, Wohlschlegel JA, Johnson PJ. Novel core promoter elements and a cognate transcription factor in the divergent unicellular eukaryote Trichomonas vaginalis. Mol Cell Biol. 2011 Apr; 31(7):1444-58. view on PubMed

Wu E, Thivierge C, Flamand M, Mathonnet G, Vashisht AA, Wohlschlegel J, Fabian MR, Sonenberg N, Duchaine TF. Pervasive and cooperative deadenylation of 3'UTRs by embryonic microRNA families. Mol Cell. 2010 Nov 24; 40(4):558-70. view on PubMed

Duch A, Palou G, Jonsson ZO, Palou R, Calvo E, Wohlschlegel J, Quintana DG. A Dbf4 mutant contributes to bypassing the Rad53-mediated block of origins of replication in response to genotoxic stress. J Biol Chem. 2011 Jan 28; 286(4):2486-91. view on PubMed

Lopez-Mosqueda J, Maas NL, Jonsson ZO, Defazio-Eli LG, Wohlschlegel J, Toczyski DP. Damage-induced phosphorylation of Sld3 is important to block late origin firing. Nature. 2010 Sep 23; 467(7314):479-83. view on PubMed

de Miguel N, Lustig G, Twu O, Chattopadhyay A, Wohlschlegel JA, Johnson PJ. Proteome analysis of the surface of Trichomonas vaginalis reveals novel proteins and strain-dependent differential expression. Mol Cell Proteomics. 2010 Jul; 9(7):1554-66. view on PubMed

Law JA, Ausin I, Johnson LM, Vashisht AA, Zhu JK, Wohlschlegel JA, Jacobsen SE. A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis. Curr Biol. 2010 May 25; 20(10):951-6. view on PubMed

Spielewoy N, Guaderrama M, Wohlschlegel JA, Ashe M, Yates JR, Wittenberg C. Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources. Eukaryot Cell. 2010 Apr; 9(4):592-601. view on PubMed

Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J, Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, Herschman HR, Clardy J, Clarke CF, Huang J. Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci U S A. 2009 Dec 22; 106(51):21984-9. view on PubMed

Konopka G, Bomar JM, Winden K, Coppola G, Jonsson ZO, Gao F, Peng S, Preuss TM, Wohlschlegel JA, Geschwind DH. Human-specific transcriptional regulation of CNS development genes by FOXP2. Nature. 2009 Nov 12; 462(7270):213-7. view on PubMed

Hernandez A, Madina BR, Ro K, Wohlschlegel JA, Willard B, Kinter MT, Cruz-Reyes J. REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding. J Biol Chem. 2010 Jan 08; 285(2):1220-8. view on PubMed

Machida YJ, Machida Y, Vashisht AA, Wohlschlegel JA, Dutta A. The deubiquitinating enzyme BAP1 regulates cell growth via interaction with HCF-1. J Biol Chem. 2009 Dec 04; 284(49):34179-88. view on PubMed

Vashisht AA, Zumbrennen KB, Huang X, Powers DN, Durazo A, Sun D, Bhaskaran N, Persson A, Uhlen M, Sangfelt O, Spruck C, Leibold EA, Wohlschlegel JA. Control of iron homeostasis by an iron-regulated ubiquitin ligase. Science. 2009 Oct 30; 326(5953):718-21. view on PubMed

Fabian MR, Mathonnet G, Sundermeier T, Mathys H, Zipprich JT, Svitkin YV, Rivas F, Jinek M, Wohlschlegel J, Doudna JA, Chen CY, Shyu AB, Yates JR, Hannon GJ, Filipowicz W, Duchaine TF, Sonenberg N. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009 Sep 24; 35(6):868-80. view on PubMed

Chuang LC, Teixeira LK, Wohlschlegel JA, Henze M, Yates JR, Méndez J, Reed SI. Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry. Mol Cell. 2009 Jul 31; 35(2):206-16. view on PubMed

Zhou R, Czech B, Brennecke J, Sachidanandam R, Wohlschlegel JA, Perrimon N, Hannon GJ. Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform. RNA. 2009 Oct; 15(10):1886-95. view on PubMed

Vagin VV, Wohlschlegel J, Qu J, Jonsson Z, Huang X, Chuma S, Girard A, Sachidanandam R, Hannon GJ, Aravin AA. Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. Genes Dev. 2009 Aug 01; 23(15):1749-62. view on PubMed

Wohlschlegel JA. Identification of SUMO-conjugated proteins and their SUMO attachment sites using proteomic mass spectrometry. Methods Mol Biol. 2009; 497:33-49. view on PubMed

Zamudio JR, Mittra B, Chattopadhyay A, Wohlschlegel JA, Sturm NR, Campbell DA. Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex. Mol Cell Biol. 2009 Mar; 29(5):1202-11. view on PubMed

Simoes-Barbosa A, Meloni D, Wohlschlegel JA, Konarska MM, Johnson PJ. Spliceosomal snRNAs in the unicellular eukaryote Trichomonas vaginalis are structurally conserved but lack a 5'-cap structure. RNA. 2008 Aug; 14(8):1617-31. view on PubMed

Reed DE, Huang XM, Wohlschlegel JA, Levine MS, Senger K. DEAF-1 regulates immunity gene expression in Drosophila. Proc Natl Acad Sci U S A. 2008 Jun 17; 105(24):8351-6. view on PubMed

Czech B, Malone CD, Zhou R, Stark A, Schlingeheyde C, Dus M, Perrimon N, Kellis M, Wohlschlegel JA, Sachidanandam R, Hannon GJ, Brennecke J. An endogenous small interfering RNA pathway in Drosophila. Nature. 2008 Jun 05; 453(7196):798-802. view on PubMed

Bellare P, Small EC, Huang X, Wohlschlegel JA, Staley JP, Sontheimer EJ. A role for ubiquitin in the spliceosome assembly pathway. Nat Struct Mol Biol. 2008 May; 15(5):444-51. view on PubMed

Olson BL, Hock MB, Ekholm-Reed S, Wohlschlegel JA, Dev KK, Kralli A, Reed SI. SCFCdc4 acts antagonistically to the PGC-1alpha transcriptional coactivator by targeting it for ubiquitin-mediated proteolysis. Genes Dev. 2008 Jan 15; 22(2):252-64. view on PubMed

Usaite R, Wohlschlegel J, Venable JD, Park SK, Nielsen J, Olsson L, Yates Iii JR. Characterization of global yeast quantitative proteome data generated from the wild-type and glucose repression saccharomyces cerevisiae strains: the comparison of two quantitative methods. J Proteome Res. 2008 Jan; 7(1):266-75. view on PubMed

Zhu W, Ukomadu C, Jha S, Senga T, Dhar SK, Wohlschlegel JA, Nutt LK, Kornbluth S, Dutta A. Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. Genes Dev. 2007 Sep 15; 21(18):2288-99. view on PubMed

Motoyama A, Xu T, Ruse CI, Wohlschlegel JA, Yates JR. Anion and cation mixed-bed ion exchange for enhanced multidimensional separations of peptides and phosphopeptides. Anal Chem. 2007 May 15; 79(10):3623-34. view on PubMed

Venable JD, Wohlschlegel J, McClatchy DB, Park SK, Yates JR. Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Anal Chem. 2007 Apr 15; 79(8):3056-64. view on PubMed

Sedgwick C, Rawluk M, Decesare J, Raithatha S, Wohlschlegel J, Semchuk P, Ellison M, Yates J, Stuart D. Saccharomyces cerevisiae Ime2 phosphorylates Sic1 at multiple PXS/T sites but is insufficient to trigger Sic1 degradation. Biochem J. 2006 Oct 01; 399(1):151-60. view on PubMed

de Bruin RA, Kalashnikova TI, Chahwan C, McDonald WH, Wohlschlegel J, Yates J, Russell P, Wittenberg C. Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback. Mol Cell. 2006 Aug; 23(4):483-96. view on PubMed

Raffa GD, Wohlschlegel J, Yates JR, Boddy MN. SUMO-binding motifs mediate the Rad60-dependent response to replicative stress and self-association. J Biol Chem. 2006 Sep 22; 281(38):27973-81. view on PubMed

Martín V, Chahwan C, Gao H, Blais V, Wohlschlegel J, Yates JR, McGowan CH, Russell P. Sws1 is a conserved regulator of homologous recombination in eukaryotic cells. EMBO J. 2006 Jun 07; 25(11):2564-74. view on PubMed

Ernkvist M, Aase K, Ukomadu C, Wohlschlegel J, Blackman R, Veitonmäki N, Bratt A, Dutta A, Holmgren L. p130-angiomotin associates to actin and controls endothelial cell shape. FEBS J. 2006 May; 273(9):2000-11. view on PubMed

Wohlschlegel JA, Johnson ES, Reed SI, Yates JR. Improved identification of SUMO attachment sites using C-terminal SUMO mutants and tailored protease digestion strategies. J Proteome Res. 2006 Apr; 5(4):761-70. view on PubMed

Pebernard S, Wohlschlegel J, McDonald WH, Yates JR, Boddy MN. The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex. Mol Cell Biol. 2006 Mar; 26(5):1617-30. view on PubMed

Duchaine TF, Wohlschlegel JA, Kennedy S, Bei Y, Conte D, Pang K, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates JR, Mello CC. Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. Cell. 2006 Jan 27; 124(2):343-54. view on PubMed

Sadygov R, Wohlschlegel J, Park SK, Xu T, Yates JR. Central limit theorem as an approximation for intensity-based scoring function. Anal Chem. 2006 Jan 01; 78(1):89-95. view on PubMed

Liu J, Rivas FV, Wohlschlegel J, Yates JR, Parker R, Hannon GJ. A role for the P-body component GW182 in microRNA function. Nat Cell Biol. 2005 Dec; 7(12):1261-6. view on PubMed

Kaiser P, Wohlschlegel J. Identification of ubiquitination sites and determination of ubiquitin-chain architectures by mass spectrometry. Methods Enzymol. 2005; 399:266-77. view on PubMed

Jónsson ZO, Jha S, Wohlschlegel JA, Dutta A. Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell. 2004 Nov 05; 16(3):465-77. view on PubMed

Venable JD, Dong MQ, Wohlschlegel J, Dillin A, Yates JR. Automated approach for quantitative analysis of complex peptide mixtures from tandem mass spectra. Nat Methods. 2004 Oct; 1(1):39-45. view on PubMed

Wohlschlegel JA, Johnson ES, Reed SI, Yates JR. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004 Oct 29; 279(44):45662-8. view on PubMed

Flick K, Ouni I, Wohlschlegel JA, Capati C, McDonald WH, Yates JR, Kaiser P. Proteolysis-independent regulation of the transcription factor Met4 by a single Lys 48-linked ubiquitin chain. Nat Cell Biol. 2004 Jul; 6(7):634-41. view on PubMed

Wohlschlegel JA, Yates JR. Proteomics: where's Waldo in yeast? Nature. 2003 Oct 16; 425(6959):671-2. view on PubMed

Wohlschlegel JA, Kutok JL, Weng AP, Dutta A. Expression of geminin as a marker of cell proliferation in normal tissues and malignancies. Am J Pathol. 2002 Jul; 161(1):267-73. view on PubMed

Wohlschlegel JA, Dhar SK, Prokhorova TA, Dutta A, Walter JC. Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45. Mol Cell. 2002 Feb; 9(2):233-40. view on PubMed

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