Hung Ton-That*
The laboratory primarily focuses on three major areas of NIH-funded research (pilus assembly, protein folding, and fusobacterial pathogenesis), aiming to elucidate the molecular mechanisms of pathogenicity by Gram-negative and Gram-positive pathogens. We employ a multidisciplinary approach combining classical and modern techniques that include bacterial genetics, various biochemical methods, electron microscopy, electron cryo-tomography, X-ray crystallography, biophysics, mass spectrometry, cell-based assays, and rodent models of infection, hence providing a rich training environment to the lab trainees.
Websites:
Hung Ton-That (Dentistry)
Ton-That Lab
Publications:
Franklin DS, Chen Y-W, Chen Y, Wittchen M, Agnew A, Luu A, Whitelegge JP, Zhou ZH, Tauch A, Das A, Ton-That H. Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence. mBio. 2024 Dec 26; e0340524. view on PubMed
Franklin DS, Chen YW, Chen Y, Wittchen M, Agnew A, Luu A, Whitelegge JP, Zhou ZH, Tauch A, Das A, Ton-That H. Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence. bioRxiv. 2024 Nov 11. view on PubMed
Chang C, Ramirez NA, Bhat AH, Nguyen MT, Kumari P, Ton-That H, Das A, Ton-That H. Biogenesis and Functionality of Sortase-Assembled Pili in Gram-Positive Bacteria. Annu Rev Microbiol. 2024 Nov; 78(1):403-423. view on PubMed
Bhat AH, Chang C, Das A, Ton-That H. Molecular basis for sortase-catalyzed pilus tip assembly. mBio. 2024 Sep 11; 15(9):e0148424. view on PubMed
Chen W, Kim SY, Lee A, Kim YJ, Chang C, Ton-That H, Kim R, Kim S, Park NH. hTERT Peptide Fragment GV1001 Prevents the Development of Porphyromonas gingivalis-Induced Periodontal Disease and Systemic Disorders in ApoE-Deficient Mice. Int J Mol Sci. 2024 Jun 01; 25(11). view on PubMed
Chang C, Ton-That H, Osipiuk J, Joachimiak A, Das A, Ton-That H. Molecular basis for dual functions in pilus assembly modulated by the lid of a pilus-specific sortase. J Biol Chem. 2024 Jun; 300(6):107329. view on PubMed
Chang C, Ton-That H. Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy. Methods Mol Biol. 2024; 2727:145-152. view on PubMed
Bhat AH, Das A, Ton-That H. Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris. Methods Mol Biol. 2024; 2727:125-134. view on PubMed
To K, Britton T, Ton-That H. Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy. Methods Mol Biol. 2024; 2727:27-33. view on PubMed
Radaic A, Kamarajan P, Cho A, Wang S, Hung GC, Najarzadegan F, Wong DT, Ton-That H, Wang CY, Kapila YL. Biological biomarkers of oral cancer. Periodontol 2000. 2023 Dec 10. view on PubMed
Britton TA, Wu C, Chen Y-W, Franklin D, Chen Y, Camacho MI, Luong TT, Das A, Ton-That H. The respiratory enzyme complex Rnf is vital for metabolic adaptation and virulence in Fusobacterium nucleatum. mBio. 2024 Jan 16; 15(1):e0175123. view on PubMed
Sue CK, Cheung NA, Mahoney BJ, McConnell SA, Scully JM, Fu JY, Chang C, Ton-That H, Loo JA, Clubb RT. The basal and major pilins in the Corynebacterium diphtheriae SpaA pilus adopt similar structures that competitively react with the pilin polymerase. Biopolymers. 2024 Jan; 115(1):e23539. view on PubMed
Reardon-Robinson ME, Nguyen MT, Sanchez BC, Osipiuk J, Rückert C, Chang C, Chen B, Nagvekar R, Joachimiak A, Tauch A, Das A, Ton-That H. A cryptic oxidoreductase safeguards oxidative protein folding in Corynebacterium diphtheriae. Proc Natl Acad Sci U S A. 2023 02 21; 120(8):e2208675120. view on PubMed
Chen YW, Camacho MI, Chen Y, Bhat AH, Chang C, Peluso EA, Wu C, Das A, Ton-That H. Genetic Determinants of Hydrogen Sulfide Biosynthesis in Fusobacterium nucleatum Are Required for Bacterial Fitness, Antibiotic Sensitivity, and Virulence. mBio. 2022 10 26; 13(5):e0193622. view on PubMed
Ramirez NA, Wu C, Chang C, Siegel SD, Das A, Ton-That H. A conserved signal-peptidase antagonist modulates membrane homeostasis of actinobacterial sortase critical for surface morphogenesis. Proc Natl Acad Sci U S A. 2022 07 12; 119(28):e2203114119. view on PubMed
Kim S, Bando Y, Chang C, Kwon J, Tarverti B, Kim D, Lee SH, Ton-That H, Kim R, Nara PL, Park NH. Topical application of Porphyromonas gingivalis into the gingival pocket in mice leads to chronic‑active infection, periodontitis and systemic inflammation. Int J Mol Med. 2022 08; 50(2). view on PubMed
Scheible M, Nguyen CT, Luong TT, Lee JH, Chen YW, Chang C, Wittchen M, Camacho MI, Tiner BL, Wu C, Tauch A, Das A, Ton-That H. The Fused Methionine Sulfoxide Reductase MsrAB Promotes Oxidative Stress Defense and Bacterial Virulence in Fusobacterium nucleatum. mBio. 2022 06 28; 13(3):e0302221. view on PubMed
Al Mamun AAM, Wu C, Chang C, Sanchez BC, Das A, Ton-That H. A cell wall-anchored glycoprotein confers resistance to cation stress in Actinomyces oris biofilms. Mol Oral Microbiol. 2022 10; 37(5):206-217. view on PubMed
Wu C, Chen YW, Scheible M, Chang C, Wittchen M, Lee JH, Luong TT, Tiner BL, Tauch A, Das A, Ton-That H. Genetic and molecular determinants of polymicrobial interactions in Fusobacterium nucleatum. Proc Natl Acad Sci U S A. 2021 06 08; 118(23). view on PubMed
McConnell SA, McAllister RA, Amer BR, Mahoney BJ, Sue CK, Chang C, Ton-That H, Clubb RT. Sortase-assembled pili in Corynebacterium diphtheriae are built using a latch mechanism. Proc Natl Acad Sci U S A. 2021 03 23; 118(12). view on PubMed
Bhat AH, Nguyen MT, Das A, Ton-That H. Anchoring surface proteins to the bacterial cell wall by sortase enzymes: how it started and what we know now. Curr Opin Microbiol. 2021 04; 60:73-79. view on PubMed
Luong TT, Nguyen MT, Chen YW, Chang C, Lee JH, Wittchen M, Ton-That H, Cruz M, Garsin DA, Das A, Tauch A, Ton-That H. Ribonuclease J-Mediated mRNA Turnover Modulates Cell Shape, Metabolism and Virulence in Corynebacterium diphtheriae. Microorganisms. 2021 Feb 14; 9(2). view on PubMed
Chen YW, Ton-That H. Corynebacterium diphtheriae Virulence Analyses Using a Caenorhabditis elegans Model. Curr Protoc Microbiol. 2020 09; 58(1):e109. view on PubMed
Chang C, Nguyen MT, Ton-That H. Genetic Manipulation of Corynebacterium diphtheriae and Other Corynebacterium Species. Curr Protoc Microbiol. 2020 09; 58(1):e111. view on PubMed
Manne K, Chattopadhyay D, Agarwal V, Blom AM, Khare B, Chakravarthy S, Chang C, Ton-That H, Narayana SVL. Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin. Acta Crystallogr D Struct Biol. 2020 Aug 01; 76(Pt 8):759-770. view on PubMed
Peluso EA, Scheible M, Ton-That H, Wu C. Genetic Manipulation and Virulence Assessment of Fusobacterium nucleatum. Curr Protoc Microbiol. 2020 06; 57(1):e104. view on PubMed
Ramirez NA, Das A, Ton-That H. New Paradigms of Pilus Assembly Mechanisms in Gram-Positive Actinobacteria. Trends Microbiol. 2020 12; 28(12):999-1009. view on PubMed
Sue CK, McConnell SA, Ellis-Guardiola K, Muroski JM, McAllister RA, Yu J, Alvarez AI, Chang C, Ogorzalek Loo RR, Loo JA, Ton-That H, Clubb RT. Kinetics and Optimization of the Lysine-Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae. Bioconjug Chem. 2020 06 17; 31(6):1624-1634. view on PubMed
Gosschalk JE, Chang C, Sue CK, Siegel SD, Wu C, Kattke MD, Yi SW, Damoiseaux R, Jung ME, Ton-That H, Clubb RT. A Cell-based Screen in Actinomyces oris to Identify Sortase Inhibitors. Sci Rep. 2020 05 22; 10(1):8520. view on PubMed
Khan A, Davlieva M, Panesso D, Rincon S, Miller WR, Diaz L, Reyes J, Cruz MR, Pemberton O, Nguyen AH, Siegel SD, Planet PJ, Narechania A, Latorre M, Rios R, Singh KV, Ton-That H, Garsin DA, Tran TT, Shamoo Y, Arias CA. Antimicrobial sensing coupled with cell membrane remodeling mediates antibiotic resistance and virulence in Enterococcus faecalis. Proc Natl Acad Sci U S A. 2019 Dec 26; 116(52):26925-26932. view on PubMed
Chang C, Wu C, Osipiuk J, Siegel SD, Zhu S, Liu X, Joachimiak A, Clubb RT, Das A, Ton-That H. Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes. Proc Natl Acad Sci U S A. 2019 09 03; 116(36):18041-18049. view on PubMed
Siegel SD, Amer BR, Wu C, Sawaya MR, Gosschalk JE, Clubb RT, Ton-That H. Erratum for Siegel et al., "Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein". mBio. 2019 04 16; 10(2). view on PubMed
Siegel SD, Amer BR, Wu C, Sawaya MR, Gosschalk JE, Clubb RT, Ton-That H. Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein. mBio. 2019 02 19; 10(1). view on PubMed
McConnell SA, Amer BR, Muroski J, Fu J, Chang C, Ogorzalek Loo RR, Loo JA, Osipiuk J, Ton-That H, Clubb RT. Protein Labeling via a Specific Lysine-Isopeptide Bond Using the Pilin Polymerizing Sortase from Corynebacterium diphtheriae. J Am Chem Soc. 2018 07 11; 140(27):8420-8423. view on PubMed
Chang C, Amer BR, Osipiuk J, McConnell SA, Huang IH, Hsieh V, Fu J, Nguyen HH, Muroski J, Flores E, Ogorzalek Loo RR, Loo JA, Putkey JA, Joachimiak A, Das A, Clubb RT, Ton-That H. In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking. Proc Natl Acad Sci U S A. 2018 06 12; 115(24):E5477-E5486. view on PubMed
Wu C, Al Mamun AAM, Luong TT, Hu B, Gu J, Lee JH, D'Amore M, Das A, Ton-That H. Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC. mBio. 2018 04 24; 9(2). view on PubMed
Luong TT, Tirgar R, Reardon-Robinson ME, Joachimiak A, Osipiuk J, Ton-That H. Structural Basis of a Thiol-Disulfide Oxidoreductase in the Hedgehog-Forming Actinobacterium Corynebacterium matruchotii. J Bacteriol. 2018 05 01; 200(9). view on PubMed
Wittchen M, Busche T, Gaspar AH, Lee JH, Ton-That H, Kalinowski J, Tauch A. Transcriptome sequencing of the human pathogen Corynebacterium diphtheriae NCTC 13129 provides detailed insights into its transcriptional landscape and into DtxR-mediated transcriptional regulation. BMC Genomics. 2018 01 25; 19(1):82. view on PubMed
Sanchez BC, Chang C, Wu C, Tran B, Ton-That H. Electron Transport Chain Is Biochemically Linked to Pilus Assembly Required for Polymicrobial Interactions and Biofilm Formation in the Gram-Positive Actinobacterium Actinomyces oris. mBio. 2017 06 20; 8(3). view on PubMed
Luong TT, Reardon-Robinson ME, Siegel SD, Ton-That H. Reoxidation of the Thiol-Disulfide Oxidoreductase MdbA by a Bacterial Vitamin K Epoxide Reductase in the Biofilm-Forming Actinobacterium Actinomyces oris. J Bacteriol. 2017 05 15; 199(10). view on PubMed
Juárez-Vázquez AL, Edirisinghe JN, Verduzco-Castro EA, Michalska K, Wu C, Noda-García L, Babnigg G, Endres M, Medina-Ruíz S, Santoyo-Flores J, Carrillo-Tripp M, Ton-That H, Joachimiak A, Henry CS, Barona-Gómez F. Evolution of substrate specificity in a retained enzyme driven by gene loss. Elife. 2017 03 31; 6. view on PubMed
Siegel SD, Reardon ME, Ton-That H. Anchoring of LPXTG-Like Proteins to the Gram-Positive Cell Wall Envelope. Curr Top Microbiol Immunol. 2017; 404:159-175. view on PubMed
Siegel SD, Liu J, Ton-That H. Biogenesis of the Gram-positive bacterial cell envelope. Curr Opin Microbiol. 2016 12; 34:31-37. view on PubMed
Siegel SD, Wu C, Ton-That H. A Type I Signal Peptidase Is Required for Pilus Assembly in the Gram-Positive, Biofilm-Forming Bacterium Actinomyces oris. J Bacteriol. 2016 08 01; 198(15):2064-73. view on PubMed
Montealegre MC, Singh KV, Somarajan SR, Yadav P, Chang C, Spencer R, Sillanpää J, Ton-That H, Murray BE. Role of the Emp Pilus Subunits of Enterococcus faecium in Biofilm Formation, Adherence to Host Extracellular Matrix Components, and Experimental Infection. Infect Immun. 2016 05; 84(5):1491-1500. view on PubMed
Echelman DJ, Alegre-Cebollada J, Badilla CL, Chang C, Ton-That H, Fernández JM. CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks. Proc Natl Acad Sci U S A. 2016 Mar 01; 113(9):2490-5. view on PubMed
Wu C, Reardon-Robinson ME, Ton-That H. Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant. Methods Mol Biol. 2016; 1440:109-22. view on PubMed
Reardon-Robinson ME, Ton-That H. Disulfide-Bond-Forming Pathways in Gram-Positive Bacteria. J Bacteriol. 2015 Dec 07; 198(5):746-54. view on PubMed
Reardon-Robinson ME, Osipiuk J, Jooya N, Chang C, Joachimiak A, Das A, Ton-That H. A thiol-disulfide oxidoreductase of the Gram-positive pathogen Corynebacterium diphtheriae is essential for viability, pilus assembly, toxin production and virulence. Mol Microbiol. 2015 Dec; 98(6):1037-50. view on PubMed
Reardon-Robinson ME, Osipiuk J, Chang C, Wu C, Jooya N, Joachimiak A, Das A, Ton-That H. A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris. J Biol Chem. 2015 Aug 28; 290(35):21393-405. view on PubMed
Galloway-Peña JR, Liang X, Singh KV, Yadav P, Chang C, La Rosa SL, Shelburne S, Ton-That H, Höök M, Murray BE. The identification and functional characterization of WxL proteins from Enterococcus faecium reveal surface proteins involved in extracellular matrix interactions. J Bacteriol. 2015 Mar; 197(5):882-92. view on PubMed
Wu C, Huang IH, Chang C, Reardon-Robinson ME, Das A, Ton-That H. Lethality of sortase depletion in Actinomyces oris caused by excessive membrane accumulation of a surface glycoprotein. Mol Microbiol. 2014 Dec; 94(6):1227-41. view on PubMed
Kang HJ, Paterson NG, Kim CU, Middleditch M, Chang C, Ton-That H, Baker EN. A slow-forming isopeptide bond in the structure of the major pilin SpaD from Corynebacterium diphtheriae has implications for pilus assembly. Acta Crystallogr D Biol Crystallogr. 2014 May; 70(Pt 5):1190-201. view on PubMed
Reardon-Robinson ME, Wu C, Mishra A, Chang C, Bier N, Das A, Ton-That H. Pilus hijacking by a bacterial coaggregation factor critical for oral biofilm development. Proc Natl Acad Sci U S A. 2014 Mar 11; 111(10):3835-40. view on PubMed
Sillanpää J, Chang C, Singh KV, Montealegre MC, Nallapareddy SR, Harvey BR, Ton-That H, Murray BE. Contribution of individual Ebp Pilus subunits of Enterococcus faecalis OG1RF to pilus biogenesis, biofilm formation and urinary tract infection. PLoS One. 2013; 8(7):e68813. view on PubMed
Broadway MM, Rogers EA, Chang C, Huang IH, Dwivedi P, Yildirim S, Schmitt MP, Das A, Ton-That H. Pilus gene pool variation and the virulence of Corynebacterium diphtheriae clinical isolates during infection of a nematode. J Bacteriol. 2013 Aug; 195(16):3774-83. view on PubMed
Krishnan V, Dwivedi P, Kim BJ, Samal A, Macon K, Ma X, Mishra A, Doran KS, Ton-That H, Narayana SV. Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions. Acta Crystallogr D Biol Crystallogr. 2013 Jun; 69(Pt 6):1073-89. view on PubMed
Chang C, Huang IH, Hendrickx AP, Ton-That H. Visualization of Gram-positive bacterial pili. Methods Mol Biol. 2013; 966:77-95. view on PubMed
Trost E, Blom J, Soares Sde C, Huang IH, Al-Dilaimi A, Schröder J, Jaenicke S, Dorella FA, Rocha FS, Miyoshi A, Azevedo V, Schneider MP, Silva A, Camello TC, Sabbadini PS, Santos CS, Santos LS, Hirata R, Mattos-Guaraldi AL, Efstratiou A, Schmitt MP, Ton-That H, Tauch A. Pangenomic study of Corynebacterium diphtheriae that provides insights into the genomic diversity of pathogenic isolates from cases of classical diphtheria, endocarditis, and pneumonia. J Bacteriol. 2012 Jun; 194(12):3199-215. view on PubMed
Wu C, Mishra A, Reardon ME, Huang IH, Counts SC, Das A, Ton-That H. Structural determinants of Actinomyces sortase SrtC2 required for membrane localization and assembly of type 2 fimbriae for interbacterial coaggregation and oral biofilm formation. J Bacteriol. 2012 May; 194(10):2531-9. view on PubMed
Khare B, Fu ZQ, Huang IH, Ton-That H, Narayana SV. The crystal structure analysis of group B Streptococcus sortase C1: a model for the "lid" movement upon substrate binding. J Mol Biol. 2011 Dec 09; 414(4):563-77. view on PubMed
Khare B, Krishnan V, Rajashankar KR, I-Hsiu H, Xin M, Ton-That H, Narayana SV. Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases: SrtA and SrtC1. PLoS One. 2011; 6(8):e22995. view on PubMed
Mishra A, Devarajan B, Reardon ME, Dwivedi P, Krishnan V, Cisar JO, Das A, Narayana SV, Ton-That H. Two autonomous structural modules in the fimbrial shaft adhesin FimA mediate Actinomyces interactions with streptococci and host cells during oral biofilm development. Mol Microbiol. 2011 Sep; 81(5):1205-20. view on PubMed
Wu C, Mishra A, Yang J, Cisar JO, Das A, Ton-That H. Dual function of a tip fimbrillin of Actinomyces in fimbrial assembly and receptor binding. J Bacteriol. 2011 Jul; 193(13):3197-206. view on PubMed
Vengadesan K, Ma X, Dwivedi P, Ton-That H, Narayana SV. A model for group B Streptococcus pilus type 1: the structure of a 35-kDa C-terminal fragment of the major pilin GBS80. J Mol Biol. 2011 Apr 15; 407(5):731-43. view on PubMed
Chang C, Mandlik A, Das A, Ton-That H. Cell surface display of minor pilin adhesins in the form of a simple heterodimeric assembly in Corynebacterium diphtheriae. Mol Microbiol. 2011 Mar; 79(5):1236-47. view on PubMed
Rogers EA, Das A, Ton-That H. Adhesion by pathogenic corynebacteria. Adv Exp Med Biol. 2011; 715:91-103. view on PubMed
Vengadesan K, Ma X, Dwivedi P, Ton-That H, Narayana SV. Purification, crystallization and halide phasing of a Streptococcus agalactiae backbone pilin GBS80 fragment. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 01; 66(Pt 12):1666-9. view on PubMed
Khare B, Samal A, Vengadesan K, Rajashankar KR, Ma X, Huang IH, Ton-That H, Narayana SV. Preliminary crystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 01; 66(Pt 9):1096-100. view on PubMed
Wu C, Ton-That H. Allelic exchange in Actinomyces oris with mCherry fluorescence counterselection. Appl Environ Microbiol. 2010 Sep; 76(17):5987-9. view on PubMed
Sillanpää J, Nallapareddy SR, Singh KV, Prakash VP, Fothergill T, Ton-That H, Murray BE. Characterization of the ebp(fm) pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection. Virulence. 2010 Jul-Aug; 1(4):236-46. view on PubMed
Mishra A, Wu C, Yang J, Cisar JO, Das A, Ton-That H. The Actinomyces oris type 2 fimbrial shaft FimA mediates co-aggregation with oral streptococci, adherence to red blood cells and biofilm development. Mol Microbiol. 2010 Aug; 77(4):841-54. view on PubMed
Kang HJ, Paterson NG, Gaspar AH, Ton-That H, Baker EN. The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds. Proc Natl Acad Sci U S A. 2009 Oct 06; 106(40):16967-71. view on PubMed
Guttilla IK, Gaspar AH, Swierczynski A, Swaminathan A, Dwivedi P, Das A, Ton-That H. Acyl enzyme intermediates in sortase-catalyzed pilus morphogenesis in gram-positive bacteria. J Bacteriol. 2009 Sep; 191(18):5603-12. view on PubMed
Mandlik A, Das A, Ton-That H. The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria. Proc Natl Acad Sci U S A. 2008 Sep 16; 105(37):14147-52. view on PubMed
Mandlik A, Swierczynski A, Das A, Ton-That H. Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm development. Trends Microbiol. 2008 Jan; 16(1):33-40. view on PubMed
Swaminathan A, Mandlik A, Swierczynski A, Gaspar A, Das A, Ton-That H. Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae. Mol Microbiol. 2007 Nov; 66(4):961-74. view on PubMed
Krishnan V, Gaspar AH, Ye N, Mandlik A, Ton-That H, Narayana SV. An IgG-like domain in the minor pilin GBS52 of Streptococcus agalactiae mediates lung epithelial cell adhesion. Structure. 2007 Aug; 15(8):893-903. view on PubMed
Mandlik A, Swierczynski A, Das A, Ton-That H. Corynebacterium diphtheriae employs specific minor pilins to target human pharyngeal epithelial cells. Mol Microbiol. 2007 Apr; 64(1):111-24. view on PubMed
Mishra A, Das A, Cisar JO, Ton-That H. Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii. J Bacteriol. 2007 Apr; 189(8):3156-65. view on PubMed
Swierczynski A, Ton-That H. Type III pilus of corynebacteria: Pilus length is determined by the level of its major pilin subunit. J Bacteriol. 2006 Sep; 188(17):6318-25. view on PubMed
Gaspar AH, Ton-That H. Assembly of distinct pilus structures on the surface of Corynebacterium diphtheriae. J Bacteriol. 2006 Feb; 188(4):1526-33. view on PubMed
Gaspar AH, Marraffini LA, Glass EM, Debord KL, Ton-That H, Schneewind O. Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope. J Bacteriol. 2005 Jul; 187(13):4646-55. view on PubMed
Ton-That H, Marraffini LA, Schneewind O. Protein sorting to the cell wall envelope of Gram-positive bacteria. Biochim Biophys Acta. 2004 Nov 11; 1694(1-3):269-78. view on PubMed
Goss JW, Sorg JA, Ramamurthi KS, Ton-That H, Schneewind O. The secretion signal of YopN, a regulatory protein of the Yersinia enterocolitica type III secretion pathway. J Bacteriol. 2004 Sep; 186(18):6320-4. view on PubMed
Marraffini LA, Ton-That H, Zong Y, Narayana SV, Schneewind O. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A. J Biol Chem. 2004 Sep 03; 279(36):37763-70. view on PubMed
Ton-That H, Marraffini LA, Schneewind O. Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae. Mol Microbiol. 2004 Jul; 53(1):251-61. view on PubMed
Ton-That H, Schneewind O. Assembly of pili in Gram-positive bacteria. Trends Microbiol. 2004 May; 12(5):228-34. view on PubMed
Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SV. Crystal structures of Staphylococcus aureus sortase A and its substrate complex. J Biol Chem. 2004 Jul 23; 279(30):31383-9. view on PubMed
Ton-That H, Schneewind O. Assembly of pili on the surface of Corynebacterium diphtheriae. Mol Microbiol. 2003 Nov; 50(4):1429-38. view on PubMed
Perry AM, Ton-That H, Mazmanian SK, Schneewind O. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring. J Biol Chem. 2002 May 03; 277(18):16241-8. view on PubMed
Mazmanian SK, Ton-That H, Su K, Schneewind O. An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis. Proc Natl Acad Sci U S A. 2002 Feb 19; 99(4):2293-8. view on PubMed
Ton-That H, Mazmanian SK, Alksne L, Schneewind O. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis. J Biol Chem. 2002 Mar 01; 277(9):7447-52. view on PubMed
Mazmanian SK, Ton-That H, Schneewind O. Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus. Mol Microbiol. 2001 Jun; 40(5):1049-57. view on PubMed
Ilangovan U, Ton-That H, Iwahara J, Schneewind O, Clubb RT. Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2001 May 22; 98(11):6056-61. view on PubMed
Ilangovan U, Iwahara J, Ton-That H, Schneewind O, Clubb RT. Assignment of the 1H, 13C and 15N signals of Sortase. J Biomol NMR. 2001 Apr; 19(4):379-80. view on PubMed
Ton-That H, Mazmanian SK, Faull KF, Schneewind O. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrates. J Biol Chem. 2000 Mar 31; 275(13):9876-81. view on PubMed
Ton-That H, Liu G, Mazmanian SK, Faull KF, Schneewind O. Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif. Proc Natl Acad Sci U S A. 1999 Oct 26; 96(22):12424-9. view on PubMed
Ton-That H, Schneewind O. Anchor structure of staphylococcal surface proteins. IV. Inhibitors of the cell wall sorting reaction. J Biol Chem. 1999 Aug 20; 274(34):24316-20. view on PubMed
Mazmanian SK, Liu G, Ton-That H, Schneewind O. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science. 1999 Jul 30; 285(5428):760-3. view on PubMed
Navarre WW, Ton-That H, Faull KF, Schneewind O. Multiple enzymatic activities of the murein hydrolase from staphylococcal phage phi11. Identification of a D-alanyl-glycine endopeptidase activity. J Biol Chem. 1999 May 28; 274(22):15847-56. view on PubMed
Navarre WW, Ton-That H, Faull KF, Schneewind O. Anchor structure of staphylococcal surface proteins. II. Cooh-terminal structure of muramidase and amidase-solubilized surface protein. J Biol Chem. 1998 Oct 30; 273(44):29135-42. view on PubMed
Ton-That H, Labischinski H, Berger-Bächi B, Schneewind O. Anchor structure of staphylococcal surface proteins. III. Role of the FemA, FemB, and FemX factors in anchoring surface proteins to the bacterial cell wall. J Biol Chem. 1998 Oct 30; 273(44):29143-9. view on PubMed
Ton-That H, Faull KF, Schneewind O. Anchor structure of staphylococcal surface proteins. A branched peptide that links the carboxyl terminus of proteins to the cell wall. J Biol Chem. 1997 Aug 29; 272(35):22285-92. view on PubMed
