Peter Bradley*
Websites:
Publications:
Pasquarelli RR, Sha J, Wohlschlegel JA, Bradley PJ. BCC0 collaborates with IMC32 and IMC43 to form the Toxoplasma gondii essential daughter bud assembly complex. PLoS Pathog. 2024 Jul; 20(7):e1012411. view on PubMed
Pasquarelli RR, Quan JJ, Cheng ES, Yang V, Britton TA, Sha J, Wohlschlegel JA, Bradley PJ. Characterization and functional analysis of Toxoplasma Golgi-associated proteins identified by proximity labelling. bioRxiv. 2024 May 22. view on PubMed
Quan JJ, Nikolov LA, Sha J, Wohlschlegel JA, Coppens I, Bradley PJ. Systematic characterization of all Toxoplasma gondii TBC domain-containing proteins identifies an essential regulator of Rab2 in the secretory pathway. PLoS Biol. 2024 May; 22(5):e3002634. view on PubMed
Pasquarelli RR, Back PS, Sha J, Wohlschlegel JA, Bradley PJ. Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii. PLoS Pathog. 2023 Oct; 19(10):e1011707. view on PubMed
Thind AC, Mota CM, Gonçalves APN, Sha J, Wohlschlegel JA, Mineo TWP, Bradley PJ. The Toxoplasma gondii effector GRA83 modulates the host's innate immune response to regulate parasite infection. mSphere. 2023 10 24; 8(5):e0026323. view on PubMed
Back PS, Senthilkumar V, Choi CP, Ly AM, Snyder AK, Lau JG, Ward GE, Bradley PJ. The Toxoplasma subpellicular network is highly interconnected and defines parasite shape for efficient motility and replication. bioRxiv. 2023 Aug 10. view on PubMed
Thind AC, Mota CM, Gonçalves APN, Sha J, Wohlschlegel JA, Mineo TWP, Bradley PJ. The Toxoplasma gondii effector GRA83 modulates the host's innate immune response to regulate parasite infection. bioRxiv. 2023 Jun 01. view on PubMed
Quan JJ, Nikolov LA, Sha J, Wohlschlegel JA, Bradley PJ. Toxoplasma gondii encodes an array of TBC-domain containing proteins including an essential regulator that targets Rab2 in the secretory pathway. bioRxiv. 2023 May 29. view on PubMed
Back PS, Moon AS, Pasquarelli RR, Bell HN, Torres JA, Chen AL, Sha J, Vashisht AA, Wohlschlegel JA, Bradley PJ. IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome. mBio. 2023 02 28; 14(1):e0304222. view on PubMed
Oliveira Souza RO, Jacobs KN, Back PS, Bradley PJ, Arrizabalaga G. IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii. J Cell Sci. 2022 11 15; 135(22). view on PubMed
Mineo TWP, Chern JH, Thind AC, Mota CM, Nadipuram SM, Torres JA, Bradley PJ. Efficient Gene Knockout and Knockdown Systems in Neospora caninum Enable Rapid Discovery and Functional Assessment of Novel Proteins. mSphere. 2022 02 23; 7(1):e0089621. view on PubMed
Chern JH, Pasquarelli RR, Moon AS, Chen AL, Sha J, Wohlschlegel JA, Bradley PJ. A Novel Toxoplasma Inner Membrane Complex Suture-Associated Protein Regulates Suture Protein Targeting and Colocalizes with Membrane Trafficking Machinery. mBio. 2021 10 26; 12(5):e0245521. view on PubMed
Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Reese ML, Bradley PJ. Multivalent Interactions Drive the Toxoplasma AC9:AC10:ERK7 Complex To Concentrate ERK7 in the Apical Cap. mBio. 2021 02 22; 13(1):e0286421. view on PubMed
Torres JA, Pasquarelli RR, Back PS, Moon AS, Bradley PJ. Identification and Molecular Dissection of IMC32, a Conserved Toxoplasma Inner Membrane Complex Protein That Is Essential for Parasite Replication. mBio. 2021 02 16; 12(1). view on PubMed
Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Hu X, Sha J, Wohlschlegel JA, Bradley PJ, Reese ML. Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis. Proc Natl Acad Sci U S A. 2020 06 02; 117(22):12164-12173. view on PubMed
Nadipuram SM, Thind AC, Rayatpisheh S, Wohlschlegel JA, Bradley PJ. Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites. PLoS One. 2020; 15(5):e0232552. view on PubMed
Bradley PJ, Rayatpisheh S, Wohlschlegel JA, Nadipuram SM. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii. Methods Mol Biol. 2020; 2071:323-346. view on PubMed
Choi CP, Moon AS, Back PS, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel JA, Bradley PJ. A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex. PLoS Biol. 2019 10; 17(10):e3000475. view on PubMed
Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry-Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B, Boulanger MJ, Lebrun M. A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites. Nat Commun. 2019 09 06; 10(1):4041. view on PubMed
Mota CM, Oliveira ACM, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TWP. Corrigendum: Neospora Caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity. Front Microbiol. 2019; 10:548. view on PubMed
Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. Retraction Note: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins. Sci Rep. 2018 07 13; 8(1):10964. view on PubMed
Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. New molecular tools in Neospora caninum for studying apicomplexan parasite proteins. Sci Rep. 2017 06 19; 7(1):3768. view on PubMed
Chen AL, Moon AS, Bell HN, Huang AS, Vashisht AA, Toh JY, Lin AH, Nadipuram SM, Kim EW, Choi CP, Wohlschlegel JA, Bradley PJ. Novel insights into the composition and function of the Toxoplasma IMC sutures. Cell Microbiol. 2017 04; 19(4). view on PubMed
Mota CM, Oliveira AC, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TW. Neospora caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity. Front Microbiol. 2016; 7:1456. view on PubMed
Nadipuram SM, Kim EW, Vashisht AA, Lin AH, Bell HN, Coppens I, Wohlschlegel JA, Bradley PJ. In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis. mBio. 2016 08 02; 7(4). view on PubMed
Kim EW, Nadipuram SM, Tetlow AL, Barshop WD, Liu PT, Wohlschlegel JA, Bradley PJ. The Rhoptry Pseudokinase ROP54 Modulates Toxoplasma gondii Virulence and Host GBP2 Loading. mSphere. 2016 Mar-Apr; 1(2). view on PubMed
Wang K, Peng ED, Huang AS, Xia D, Vermont SJ, Lentini G, Lebrun M, Wastling JM, Bradley PJ. Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Vicia villosa Lectin Chromatography. PLoS One. 2016; 11(3):e0150561. view on PubMed
Silmon de Monerri NC, Yakubu RR, Chen AL, Bradley PJ, Nieves E, Weiss LM, Kim K. The Ubiquitin Proteome of Toxoplasma gondii Reveals Roles for Protein Ubiquitination in Cell-Cycle Transitions. Cell Host Microbe. 2015 Nov 11; 18(5):621-33. view on PubMed
Rodriguez JA, Xu R, Chen CC, Huang Z, Jiang H, Chen AL, Raines KS, Pryor A, Nam D, Wiegart L, Song C, Madsen A, Chushkin Y, Zontone F, Bradley PJ, Miao J. Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells. IUCrJ. 2015 Sep 01; 2(Pt 5):575-83. view on PubMed
Gold DA, Kaplan AD, Lis A, Bett GC, Rosowski EE, Cirelli KM, Bougdour A, Sidik SM, Beck JR, Lourido S, Egea PF, Bradley PJ, Hakimi MA, Rasmusson RL, Saeij JP. The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole. Cell Host Microbe. 2015 May 13; 17(5):642-52. view on PubMed
Chen AL, Kim EW, Toh JY, Vashisht AA, Rashoff AQ, Van C, Huang AS, Moon AS, Bell HN, Bentolila LA, Wohlschlegel JA, Bradley PJ. Novel components of the Toxoplasma inner membrane complex revealed by BioID. mBio. 2015 Feb 17; 6(1):e02357-14. view on PubMed
Tonkin ML, Beck JR, Bradley PJ, Boulanger MJ. The inner membrane complex sub-compartment proteins critical for replication of the apicomplexan parasite Toxoplasma gondii adopt a pleckstrin homology fold. J Biol Chem. 2014 May 16; 289(20):13962-73. view on PubMed
Beck JR, Chen AL, Kim EW, Bradley PJ. RON5 is critical for organization and function of the Toxoplasma moving junction complex. PLoS Pathog. 2014 Mar; 10(3):e1004025. view on PubMed
Child MA, Hall CI, Beck JR, Ofori LO, Albrow VE, Garland M, Bowyer PW, Bradley PJ, Powers JC, Boothroyd JC, Weerapana E, Bogyo M. Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion. Nat Chem Biol. 2013 Oct; 9(10):651-6. view on PubMed
de Leon JC, Scheumann N, Beatty W, Beck JR, Tran JQ, Yau C, Bradley PJ, Gull K, Wickstead B, Morrissette NS. A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components. Eukaryot Cell. 2013 Jul; 12(7):1009-19. view on PubMed
Beck JR, Fung C, Straub KW, Coppens I, Vashisht AA, Wohlschlegel JA, Bradley PJ. A Toxoplasma palmitoyl acyl transferase and the palmitoylated armadillo repeat protein TgARO govern apical rhoptry tethering and reveal a critical role for the rhoptries in host cell invasion but not egress. PLoS Pathog. 2013 Feb; 9(2):e1003162. view on PubMed
Tonkin ML, Brown S, Beck JR, Bradley PJ, Boulanger MJ. Purification, crystallization and preliminary X-ray diffraction analysis of inner membrane complex (IMC) subcompartment protein 1 (ISP1) from Toxoplasma gondii. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 01; 68(Pt 7):832-4. view on PubMed
Fung C, Beck JR, Robertson SD, Gubbels MJ, Bradley PJ. Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation. Mol Biochem Parasitol. 2012 Aug; 184(2):99-108. view on PubMed
Anderson-White B, Beck JR, Chen CT, Meissner M, Bradley PJ, Gubbels MJ. Cytoskeleton assembly in Toxoplasma gondii cell division. Int Rev Cell Mol Biol. 2012; 298:1-31. view on PubMed
Hajagos BE, Turetzky JM, Peng ED, Cheng SJ, Ryan CM, Souda P, Whitelegge JP, Lebrun M, Dubremetz JF, Bradley PJ. Molecular dissection of novel trafficking and processing of the Toxoplasma gondii rhoptry metalloprotease toxolysin-1. Traffic. 2012 Feb; 13(2):292-304. view on PubMed
Liang L, Döskaya M, Juarez S, Caner A, Jasinskas A, Tan X, Hajagos BE, Bradley PJ, Korkmaz M, Gürüz Y, Felgner PL, Davies DH. Identification of potential serodiagnostic and subunit vaccine antigens by antibody profiling of toxoplasmosis cases in Turkey. Mol Cell Proteomics. 2011 Jul; 10(7):M110.006916. view on PubMed
Sohn CS, Cheng TT, Drummond ML, Peng ED, Vermont SJ, Xia D, Cheng SJ, Wastling JM, Bradley PJ. Identification of novel proteins in Neospora caninum using an organelle purification and monoclonal antibody approach. PLoS One. 2011 Apr 04; 6(4):e18383. view on PubMed
Straub KW, Peng ED, Hajagos BE, Tyler JS, Bradley PJ. The moving junction protein RON8 facilitates firm attachment and host cell invasion in Toxoplasma gondii. PLoS Pathog. 2011 Mar; 7(3):e1002007. view on PubMed
Beck JR, Rodriguez-Fernandez IA, de Leon JC, Huynh MH, Carruthers VB, Morrissette NS, Bradley PJ. A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division. PLoS Pathog. 2010 Sep 09; 6(9):e1001094. view on PubMed
Turetzky JM, Chu DK, Hajagos BE, Bradley PJ. Processing and secretion of ROP13: A unique Toxoplasma effector protein. Int J Parasitol. 2010 Aug 01; 40(9):1037-44. view on PubMed
Breinich MS, Ferguson DJ, Foth BJ, van Dooren GG, Lebrun M, Quon DV, Striepen B, Bradley PJ, Frischknecht F, Carruthers VB, Meissner M. A dynamin is required for the biogenesis of secretory organelles in Toxoplasma gondii. Curr Biol. 2009 Feb 24; 19(4):277-86. view on PubMed
Straub KW, Cheng SJ, Sohn CS, Bradley PJ. Novel components of the Apicomplexan moving junction reveal conserved and coccidia-restricted elements. Cell Microbiol. 2009 Apr; 11(4):590-603. view on PubMed
Rome ME, Beck JR, Turetzky JM, Webster P, Bradley PJ. Intervacuolar transport and unique topology of GRA14, a novel dense granule protein in Toxoplasma gondii. Infect Immun. 2008 Nov; 76(11):4865-75. view on PubMed
DeRocher AE, Coppens I, Karnataki A, Gilbert LA, Rome ME, Feagin JE, Bradley PJ, Parsons M. A thioredoxin family protein of the apicoplast periphery identifies abundant candidate transport vesicles in Toxoplasma gondii. Eukaryot Cell. 2008 Sep; 7(9):1518-29. view on PubMed
Bradley PJ, Sibley LD. Rhoptries: an arsenal of secreted virulence factors. Curr Opin Microbiol. 2007 Dec; 10(6):582-7. view on PubMed
Gilbert LA, Ravindran S, Turetzky JM, Boothroyd JC, Bradley PJ. Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells. Eukaryot Cell. 2007 Jan; 6(1):73-83. view on PubMed
Lebrun M, Michelin A, El Hajj H, Poncet J, Bradley PJ, Vial H, Dubremetz JF. The rhoptry neck protein RON4 re-localizes at the moving junction during Toxoplasma gondii invasion. Cell Microbiol. 2005 Dec; 7(12):1823-33. view on PubMed
Alexander DL, Mital J, Ward GE, Bradley P, Boothroyd JC. Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles. PLoS Pathog. 2005 Oct; 1(2):e17. view on PubMed
Bradley PJ, Ward C, Cheng SJ, Alexander DL, Coller S, Coombs GH, Dunn JD, Ferguson DJ, Sanderson SJ, Wastling JM, Boothroyd JC. Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii. J Biol Chem. 2005 Oct 07; 280(40):34245-58. view on PubMed
Bradley PJ, Li N, Boothroyd JC. A GFP-based motif-trap reveals a novel mechanism of targeting for the Toxoplasma ROP4 protein. Mol Biochem Parasitol. 2004 Sep; 137(1):111-20. view on PubMed
Bradley PJ, Hsieh CL, Boothroyd JC. Unprocessed Toxoplasma ROP1 is effectively targeted and secreted into the nascent parasitophorous vacuole. Mol Biochem Parasitol. 2002 Nov-Dec; 125(1-2):189-93. view on PubMed
